BRAIN MOLECULAR ANATOMY PROJECT: GENE DISCOVERY

基因在发育过程中的精确时空表达是 对确定大脑的结构和功能至关重要。这个 不同脑细胞中基因表达谱的测定 脑发育过程中的类型和病理生理过程中的变化 各国将提供宝贵的信息，这将有助于 对心理健康的更多了解和更好的诊断， 治疗，并最终预防严重的大脑疾病。 脑分子解剖学计划(BMAP)是一项跨领域的研究。 由NIMH和NINDS领导的多个研究所的学科努力旨在 建立最先进的技术和信息系统，以 破译大脑的分子解剖学，并由基因组成 发现和基因表达分析阶段。基因的发现 阶段，该阶段旨在对符合以下条件的基因进行分类 不同种类的神经系统细胞在不同的时间表达 发育阶段，从努力识别基因开始 用C57BL6J菌株在成年小鼠脑内表达。这份合同 将完成以下工作：(1)构造单独标记的 来自11个脑区的非归一化和归一化的cDNA文库； (2)生成11个序列减去的规格化文库 日益丰富的复杂频率类的mRNAs (稀有的mRNAs)；(3)确定超过75,000个EST序列 序列减去文库，并构造一个非冗余的 收集&gt；20,000个独特的EST，用于“老鼠”的开发 脑基因克隆和文库《脑基因克隆和文库》 集合及其关联数据将为 科学界未来在这方面的功能研究 神经系统是有基础的。

The precise spatio-temporal expression of genes during development is critical for determining the structure and function of the brain. The determination of the profile of genes expressed in various brain cell types during brain development and altered during pathophysiological states will provide valuable information that will contribute to greater understanding of mental health and the improved diagnosis, treatment, and eventually prevention of serious brain disorders. Brain Molecular Anatomy Project (BMAP), which is an inter- disciplinary, multi-institute effort led by NIMH and NINDS, aims to establish state-of-the-art technologies and informatics systems to decipher the molecular anatomy of the brain and consists of the gene discovery and gene expression analysis phases. The gene discovery phase, which aims to catalog the repertoire of genes that are expressed by different kinds of nervous system cells at different developmental stages, begins with an effort to identify genes expressed in adult mouse brain using strain C57BL6J. The contract will accomplish the following: (1) to construct individually tagged non-normalized and normalized cDNA libraries from 11 brain regions; (2) to generate 11 serially subtracted normalized libraries increasingly enriched for the mRNAs of the complex frequency class (rare mRNAs); (3) determine sequences of over 75,000 3 ESTs from serially subtracted libraries, and construct a non-redundant collection of > 20,000 unique ESTs for the development of a "mouse brain UNIGENE set". Individual cDNA clones and libraries, unigene sets, and associated data will provide invaluable resources for the scientific community upon which future functional studies in the nervous systems can be based.