NHLBI Shared Microarray Facilities

描述（由申请人提供）： 这项建议的首要目标是促进微阵列技术的应用，为爱荷华州大学的NHLBI资助的研究人员进行基础假设驱动的研究。拟议的共享微阵列设施有三个组成部分：（A）微阵列杂交实验室，（B）信息学核心实验室，和（C）统计学核心实验室。微阵列杂交实验室将负责以下活动：（a）阵列制作：来自非冗余阵列cDNA集合的cDNA插入片段的PCR扩增/纯化、载玻片打印和处理;（B）RNA纯化、靶标制备/标记、杂交和杂交后处理;（c）扫描/成像捕获、处理和分析。我们的提案包含六个与NHLBI资助的心脏和肺部疾病领域研究人员实验室相关的项目。爱荷华州大学大约有130名NHLBI研究人员，他们在心脏、肺、血液和睡眠领域可以从这些设施中受益。共享微阵列设施将为这些科学家提供广泛的个性化支持，包括研究设计，文库构建，载玻片打印，微阵列杂交结果分析，包括生物信息学和统计支持。生物信息学的支持还将确保这些研究的结果及时提供给社区。六个初始项目包括：1）发育中心脏的差异基因表达，2）室周器官的差异基因表达：维持心血管稳态的见解，3）高血压大鼠模型中的差异基因表达谱4）静息和RSV感染的气道上皮中的差异基因表达5）使用微阵列技术剖析气道上皮天然免疫应答6）CF和非CF气道上皮中的差异基因表达。

DESCRIPTION (provided by applicant): The over riding goal of this proposal is to facilitate the application of microarray technology to fundamental, hypothesis-driven research for NHLBI funded investigators at the University of Iowa. The proposed Shared Microarray Facility has three components: (A) Microarray Hybridization Laboratory, (B) Informatics Core Laboratory, and (C) Statistics Core Laboratory. The Microarray Hybridization Laboratory will be responsible for the following activities: (a) array fabrication: PCR amplification/purification of cDNA inserts from non-redundant arrayed cDNA collections, slide printing and processing; (b) RNA purification, target preparation/labeling, hybridization and post-hybridization processes, (c) scanning/imaging capturing, processing and analysis. Our proposal contains six projects linked to the laboratories of NHLBI funded investigators in the areas of heart and lung diseases. There are approximately 130 NHLBI investigators at the University of Iowa in the areas of heart, lung, blood, and sleep that could benefit from these facilities. The Shared Microarray Facility will provide these scientists with a broad range of individualized support including assistance with study design, construction of libraries, slide printing, analysis of microarray hybridization results including bioinformatic and statistical support. The bioinformatics support will also make sure that results from these studies are promptly made available to the community. The six initial projects include: 1) Differential Gene Expression in Developing Heart, 2) Differential Gene Expression in Circumventricular Organs: Insights to the Maintenance of Cardiovascular Homeostasis, 3) Differential Gene Expression Profiling in a Rat Model of Hypertension 4) Differential Gene Expression in Resting and RSV-Infected Airway Epithelia 5) Dissecting Airway Epithelial Innate Immune Responses using Microarray Technology 6) Differential Gene Expression in CF and non-CF Airway Epithelia.