BRAIN MOLECULAR ANATOMY PROJECT: GENE DISCOVERY

基因在发育过程中的精确时空表达是 对决定大脑的结构和功能至关重要。的 确定在各种脑细胞中表达的基因谱 在大脑发育过程中的类型和病理生理过程中改变 各国将提供宝贵的信息， 更好地了解心理健康和改善诊断， 治疗，并最终预防严重的脑部疾病。 脑分子解剖学项目（Brain Molecular Anatomy Project，简称BMAP），是一个跨... 由NIMH和NINDS领导的多学科、多机构的努力，旨在 建立最先进的技术和信息系统， 破译大脑的分子解剖结构， 发现和基因表达分析阶段。基因发现 阶段，其目的是编目基因的剧目， 由不同种类的神经系统细胞在不同的 在发育阶段，首先要努力识别基因 使用菌株C57 BL 6 J在成年小鼠脑中表达。合同 将完成以下任务：（1）构建单独标记的 来自11个脑区的非标准化和标准化cDNA文库; (2)以产生11个连续减去的标准化文库 复杂频率类别的mRNA越来越丰富， (rare mRNAs）;（3）测定来自 串行减法库，并构建非冗余 收集> 20，000个独特的EST用于开发“小鼠” 脑UNIGENE设置”。单个cDNA克隆和文库，单基因 集，以及相关数据将提供宝贵的资源 科学界，未来的功能研究， 神经系统的基础。

The precise spatio-temporal expression of genes during development is critical for determining the structure and function of the brain. The determination of the profile of genes expressed in various brain cell types during brain development and altered during pathophysiological states will provide valuable information that will contribute to greater understanding of mental health and the improved diagnosis, treatment, and eventually prevention of serious brain disorders. Brain Molecular Anatomy Project (BMAP), which is an inter- disciplinary, multi-institute effort led by NIMH and NINDS, aims to establish state-of-the-art technologies and informatics systems to decipher the molecular anatomy of the brain and consists of the gene discovery and gene expression analysis phases. The gene discovery phase, which aims to catalog the repertoire of genes that are expressed by different kinds of nervous system cells at different developmental stages, begins with an effort to identify genes expressed in adult mouse brain using strain C57BL6J. The contract will accomplish the following: (1) to construct individually tagged non-normalized and normalized cDNA libraries from 11 brain regions; (2) to generate 11 serially subtracted normalized libraries increasingly enriched for the mRNAs of the complex frequency class (rare mRNAs); (3) determine sequences of over 75,000 3 ESTs from serially subtracted libraries, and construct a non-redundant collection of > 20,000 unique ESTs for the development of a "mouse brain UNIGENE set". Individual cDNA clones and libraries, unigene sets, and associated data will provide invaluable resources for the scientific community upon which future functional studies in the nervous systems can be based.