NHLBI Shared Microarray Facilities

NHLBI 共享微阵列设施

• 批准号: 6785294
• 负责人: Marcelo Bento Soares
• 金额: $ 73.5万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6785294
• 关键词: RNA; bioinformatics; biological models; biomedical facility; cardiovascular system; clinical research; complementary DNA; cystic fibrosis; gene expression; gene expression profiling; heart; histogenesis; homeostasis; hypertension; image processing; imaging /visualization /scanning; immunity; microarray technology; nucleic acid hybridization; nucleic acid purification; respiratory epithelium; respiratory syncytial virus; statistics /biometry

DESCRIPTION (provided by applicant): The over riding goal of this proposal is to facilitate the application of microarray technology to fundamental, hypothesis-driven research for NHLBI funded investigators at the University of Iowa. The proposed Shared Microarray Facility has three components: (A) Microarray Hybridization Laboratory, (B) Informatics Core Laboratory, and (C) Statistics Core Laboratory. The Microarray Hybridization Laboratory will be responsible for the following activities: (a) array fabrication: PCR amplification/purification of cDNA inserts from non-redundant arrayed cDNA collections, slide printing and processing; (b) RNA purification, target preparation/labeling, hybridization and post-hybridization processes, (c) scanning/imaging capturing, processing and analysis. Our proposal contains six projects linked to the laboratories of NHLBI funded investigators in the areas of heart and lung diseases. There are approximately 130 NHLBI investigators at the University of Iowa in the areas of heart, lung, blood, and sleep that could benefit from these facilities. The Shared Microarray Facility will provide these scientists with a broad range of individualized support including assistance with study design, construction of libraries, slide printing, analysis of microarray hybridization results including bioinformatic and statistical support. The bioinformatics support will also make sure that results from these studies are promptly made available to the community. The six initial projects include: 1) Differential Gene Expression in Developing Heart, 2) Differential Gene Expression in Circumventricular Organs: Insights to the Maintenance of Cardiovascular Homeostasis, 3) Differential Gene Expression Profiling in a Rat Model of Hypertension 4) Differential Gene Expression in Resting and RSV-Infected Airway Epithelia 5) Dissecting Airway Epithelial Innate Immune Responses using Microarray Technology 6) Differential Gene Expression in CF and non-CF Airway Epithelia.

描述(由申请人提供)： 这项提案的首要目标是为爱荷华大学NHLBI资助的研究人员将微阵列技术应用于基础的、假设驱动的研究提供便利。拟议的共享微阵列设施有三个组成部分：(A)微阵列杂交实验室、(B)信息学核心实验室和(C)统计核心实验室。微阵列杂交实验室将负责以下活动：(A)阵列制造：聚合酶链式反应扩增/纯化来自非冗余阵列cDNA收集物的cDNA插入物、载玻片打印和处理；(B)核糖核酸纯化、靶标制备/标记、杂交和杂交后处理；(C)扫描/成像捕获、处理和分析。我们的提案包含与NHLBI资助的心脏和肺病领域研究人员的实验室相关的六个项目。爱荷华大学大约有130名NHLBI研究人员在心、肺、血液和睡眠领域可以从这些设施中受益。共享的微阵列设施将为这些科学家提供广泛的个性化支持，包括协助研究设计、构建文库、幻灯片打印、分析微阵列杂交结果，包括生物信息学和统计支持。生物信息学支持还将确保这些研究的结果迅速提供给社区。这六个最初的项目包括：1)发育中心脏的差异基因表达，2)体外循环器官的差异基因表达：维持心血管稳态的启示，3)高血压大鼠模型的差异基因表达谱4)静息和RSV感染的呼吸道上皮细胞的差异基因表达5)利用基因芯片技术剖析呼吸道上皮细胞的先天免疫反应6)CF和非CF呼吸道上皮细胞的差异基因表达。