STRUCTURAL BASIS FOR STIMULUS CODING IN THE COCHLEAR NUCLEUS

耳蜗核刺激编码的结构基础

• 批准号: 6201692
• 负责人: DAVID K. RYUGO
• 金额: $ 10.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201692
• 关键词: G protein; auditory nuclei; biological signal transduction; brain mapping; cats; cell type; electron microscopy; electrophysiology; evoked potentials; hearing; immunocytochemistry; intercellular connection; laboratory rat; membrane channels; neural information processing; neuroanatomy; neurochemistry; neurons; pons; single cell analysis; synapses; synaptic vesicles

Our long-term goals are to understand neuronal mechanisms that underlie hearing in mammals. In the auditory nervous system, all acoustic information from the environment enters the brain by passing from the auditory nerve and synapsing in the cochlear nucleus. In the cochlear nucleus, the relatively homogeneous responses of incoming auditory nerve fibers are transformed into a variety of different response patterns by the different classes of resident neurons, and the resultant signals are in turn transmitted to higher centers. The spectrum of these responses is hypothesized to depend upon the synaptic organization of auditory nerve input, intrinsic neurons, and descending inputs; the types and distribution of receptors, ion channels, and G proteins; and second messengers, all of which form the signaling capabilities in each cell class. In order to understand the how sound is processed at this key auditory nucleus, there is a need to study identified cell populations, to analyze their synaptic connections, and to reveal features of their signal processing capabilities. The present proposal will apply anterograde and retrograde tract tracing methods to identify neurons and their circuits, and immunocytochemical staining procedures to reveal the chemistry of these different neuron populations. In addition, we will apply in vivo and in vitro intracellular recording and staining methods in order to reveal structure-function relationships in the cochlear nucleus at the single cell level. The data from these projects will provide insights into how neurons and their circuits shape the coding process in the central auditory system, and could help in the design of a cochlear nucleus implant for deaf individuals who cannot benefit from a cochlear prothesis.

我们的长期目标是了解基于的神经元机制 在哺乳动物中听到。在听觉神经系统中，所有声学 来自环境的信息通过从 耳蜗核中的听觉神经和突触。在人工耳蜗中 核，传入的听觉神经的相对均匀反应 纤维通过 不同类别的常驻神经元以及由此产生的信号是 反过来传输到更高的中心。这些响应的范围是 假设取决于听觉神经的突触组织 输入，内在神经元和降序输入；类型和 受体，离子通道和G蛋白的分布；第二 使者，所有这些都形成了每个单元格中的信号功能 班级。为了了解在此键上处理声音的方式 听觉核，需要研究确定的细胞群体， 分析其突触连接并揭示信号的特征 处理功能。 目前的提案将采用顺行和逆行图形跟踪 识别神经元及其电路的方法以及免疫细胞化学 染色程序以揭示这些不同神经元的化学 人群。此外，我们将在体内和体外细胞内应用 记录和染色方法以揭示结构功能 单细胞水平的人工耳蜗核中的关系。数据 从这些项目中将提供有关神经元及其其如何的见解 电路在中央听觉系统中塑造编码过程，并塑造 可以帮助设计聋人的耳蜗核植入物 无法从人工耳蜗中受益的个人。