HISTOLOGY CORE

组织学核心

• 批准号: 7914233
• 负责人: DAVID K. RYUGO
• 金额: $ 28.86万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-23 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7914233
• 关键词: Behavioral; Collaborations; Computer software; Data; Data Collection; Development; Digital Photography; Educational process of instructing; Electron Microscope; Electron Microscopy; Electrons; Equilibrium; Equipment; Faculty; Fluorescence; Fostering; Foundations; Frozen Sections; Goals; Hand; Hearing; Histologic; Histology; Image; Imagery; Individual; Instruction; Investigation; Laboratories; Methods; Microanatomy; Microscope; Microtome - medical device; Mission; Molecular; Nature; Neuraxis; Neurobiology; Neurophysiology - biologic function; Neurosciences; Peripheral; Physiological; Plastics; Postdoctoral Fellow; Preparation; Procedures; Process; Research; Research Personnel; Research Project Grants; Research Technics; Resource Sharing; Resources; Science; Scientist; Sensory; Services; Smell Perception; Staging; Staining method; Stains; Structure; Techniques; Time; Tissues; Training; cost; digital; experience; graduate student; immunocytochemistry; implementation research; light microscopy; meetings; meter; new technology; sample fixation; skills; tissue preparation; tissue processing; tool

The goal of this Core is to provide faculty in the Center for Hearing and Balance, and associated laboratories, with access to histological equipment and expertise and assistance. The visualization and quantification of structural features that correlate with physiological, developmental, and/or plastic phenomena within the peripheral and central nervous systems provide fundamental data necessary to infer basic mechanisms of neural function. But with the increasingly specialized nature of science, the expense and difficulty of such studies can place them out of reach for most laboratories. Common resources foster collaborations between scientists with different skill sets and can be the key to implementing exciting research projects that bridge different levels of analysis. Anatomical data frequently support this bridge. The Histology Core will continue to provide assistance as well as access to, and instruction in the tools and resources required for tissue preparation and sectioning for light microscopy. This includes both plasticembedded and frozen sections. The Core provides and maintains a light/fluorescence compound microscope with digital camera for image acquisition and auxiliary workstations for analysis. In addition the Core will enable ultrastructural studies through the services of an experienced electron microsopist.

这个核心的目标是为听力和平衡中心提供教员，并与 实验室，有机会获得组织学设备、专门知识和援助。视觉化和 量化与生理、发育和/或塑料相关的结构特征 外周和中枢神经系统内的现象提供了推断所需的基本数据 神经功能的基本机制。但随着科学的专业性越来越强，费用 而这类研究的难度可能会使它们成为大多数实验室无法企及的。共同资源培育 拥有不同技能的科学家之间的合作可能是实施令人兴奋的 连接不同分析层次的研究项目。解剖学数据经常支持这座桥。 组织学核心将继续提供援助以及获取和指导工具和 组织准备和光学显微镜切片所需的资源。这包括嵌入塑料的 和冰冻切片。核心提供并维持光/荧光化合物 显微镜配有数码相机进行图像采集和辅助工作站进行分析。此外， CORE将通过经验丰富的电子显微操作员的服务来进行超微结构研究。