HISTOLOGY CORE

组织学核心

• 批准号: 7914233
• 负责人: DAVID K. RYUGO
• 金额: $ 28.86万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-23 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7914233
• 关键词: Behavioral; Collaborations; Computer software; Data; Data Collection; Development; Digital Photography; Educational process of instructing; Electron Microscope; Electron Microscopy; Electrons; Equilibrium; Equipment; Faculty; Fluorescence; Fostering; Foundations; Frozen Sections; Goals; Hand; Hearing; Histologic; Histology; Image; Imagery; Individual; Instruction; Investigation; Laboratories; Methods; Microanatomy; Microscope; Microtome - medical device; Mission; Molecular; Nature; Neuraxis; Neurobiology; Neurophysiology - biologic function; Neurosciences; Peripheral; Physiological; Plastics; Postdoctoral Fellow; Preparation; Procedures; Process; Research; Research Personnel; Research Project Grants; Research Technics; Resource Sharing; Resources; Science; Scientist; Sensory; Services; Smell Perception; Staging; Staining method; Stains; Structure; Techniques; Time; Tissues; Training; cost; digital; experience; graduate student; immunocytochemistry; implementation research; light microscopy; meetings; meter; new technology; sample fixation; skills; tissue preparation; tissue processing; tool

The goal of this Core is to provide faculty in the Center for Hearing and Balance, and associated laboratories, with access to histological equipment and expertise and assistance. The visualization and quantification of structural features that correlate with physiological, developmental, and/or plastic phenomena within the peripheral and central nervous systems provide fundamental data necessary to infer basic mechanisms of neural function. But with the increasingly specialized nature of science, the expense and difficulty of such studies can place them out of reach for most laboratories. Common resources foster collaborations between scientists with different skill sets and can be the key to implementing exciting research projects that bridge different levels of analysis. Anatomical data frequently support this bridge. The Histology Core will continue to provide assistance as well as access to, and instruction in the tools and resources required for tissue preparation and sectioning for light microscopy. This includes both plasticembedded and frozen sections. The Core provides and maintains a light/fluorescence compound microscope with digital camera for image acquisition and auxiliary workstations for analysis. In addition the Core will enable ultrastructural studies through the services of an experienced electron microsopist.

该核心的目标是提供听力和平衡中心的教师，以及相关的 实验室，并获得组织学设备和专业知识和援助。可视化和 与生理、发育和/或可塑性相关的结构特征的量化 外周和中枢神经系统内的现象提供了推断 神经功能的基本机制。但随着科学的日益专业化， 并且这些研究的困难使得它们对于大多数实验室来说遥不可及。共同资源促进 具有不同技能的科学家之间的合作，可以成为实现令人兴奋的 研究项目，桥梁不同层次的分析。解剖学数据经常支持这一桥梁。 组织学核心将继续提供援助，以及获得，并在工具和指导， 组织制备和光学显微镜切片所需的资源。这包括塑料嵌入式 冷冻切片核心提供并维持光/荧光化合物 用于图像采集的数码相机和用于分析的辅助工作站。此外 核心将使超微结构的研究，通过服务的经验丰富的电子显微镜。