HISTOLOGY CORE

组织学核心

基本信息

批准号：
8305649
负责人：
DAVID K. RYUGO
金额：
$ 28.99万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-23 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8305649
关键词：
Behavioral Collaborations Computer software Data Data Collection Development Digital Photography Educational process of instructing Electron Microscope Electron Microscopy Electrons Equilibrium Equipment Faculty Fluorescence Fostering Foundations Frozen Sections Goals Hearing Histologic Histology Image Imagery Individual Instruction Investigation Laboratories Light Methods Microanatomy Microscope Microtome - medical device Mission Molecular Nature Neuraxis Neurobiology Neurophysiology - biologic function Neurosciences Peripheral Physiological Plastics Postdoctoral Fellow Preparation Procedures Process Research Research Personnel Research Project Grants Research Technics Resource Sharing Resources Science Scientist Sensory Services Smell Perception Staging Staining method Stains Structure Techniques Time Tissues Training cost effective digital experience graduate student immunocytochemistry implementation research light microscopy meetings meter new technology sample fixation skills tissue preparation tissue processing tool

项目摘要

The goal of this Core is to provide faculty in the Center for Hearing and Balance, and associated laboratories, with access to histological equipment and expertise and assistance. The visualization and quantification of structural features that correlate with physiological, developmental, and/or plastic phenomena within the peripheral and central nervous systems provide fundamental data necessary to infer basic mechanisms of neural function. But with the increasingly specialized nature of science, the expense and difficulty of such studies can place them out of reach for most laboratories. Common resources foster collaborations between scientists with different skill sets and can be the key to implementing exciting research projects that bridge different levels of analysis. Anatomical data frequently support this bridge. The Histology Core will continue to provide assistance as well as access to, and instruction in the tools and resources required for tissue preparation and sectioning for light microscopy. This includes both plasticembedded and frozen sections. The Core provides and maintains a light/fluorescence compound microscope with digital camera for image acquisition and auxiliary workstations for analysis. In addition the Core will enable ultrastructural studies through the services of an experienced electron microsopist.

该核心的目的是在听力和平衡中心提供教师，并相关实验室，可以使用组织学设备以及专业知识和帮助。可视化和量化与生理，发育和/或塑料相关的结构特征周围和中枢神经系统中的现象提供了推断必要的基本数据神经功能的基本机制。但是，随着科学的越来越专业的本质，费用对于大多数实验室，此类研究的困难可能使它们无法触及。共同资源寄养科学家之间具有不同技能的合作，可能是实施令人兴奋的关键研究项目桥接不同的分析水平。解剖学数据经常支持这座桥。组织学核心将继续提供帮助以及对工具的访问和指导组织制备和切片所需的资源用于光学显微镜。这两个包括塑料床和冷冻部分。芯提供并保持光/荧光化合物具有数码相机的显微镜，用于图像采集和辅助工作站进行分析。另外核心将通过经验丰富的电子微知识者的服务来实现超微结构研究。