Biogenic amines in malaria mosquitoes: from hearing to swarming behaviour
疟疾蚊子中的生物胺:从听觉到集群行为
基本信息
- 批准号:MR/S015493/1
- 负责人:
- 金额:$ 157.66万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Fellowship
- 财政年份:2019
- 资助国家:英国
- 起止时间:2019 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Mosquitoes are more sensitive to sound than any other arthropod, by means of their fascinating tiny antennal ears, the Johnston 's organ (JO). In an organ smaller that a pin's head, male mosquitoes harbour around 15,000 auditory neurons roughly equalling the number of hair in human cochlea. Mosquitoes use their sophisticated JO's to recognize the flight tones of mating partners. Every day around sunset, male mosquitoes aggregate in areal swarms that attract females. Within swarms, male and female acoustically detect each other by their wing beats. Due to the necessity for mosquito mating, pre-mating acoustic interactions and swarming behaviour are prime targets for mosquito control tools. Despite this, the neurobiology of mosquito swarming behaviour, and the physiological mechanisms that induce it, are almost entirely unknown.We have recently shown how the mosquito JO function is modulated by an auditory efferent system. This is remarkable as auditory efferent control was believed to exist only in vertebrates. We show that the biogenic amines octopamine and serotonin are released in the mosquito JO and modulate sound-induced mechanical and electrical responses, likely contributing to JO's sophistication as sound detector. Interestingly, biogenic amines induce swarming and social behaviour in other insects. Taken together and given the intimate relationship between hearing and swarming behaviour in mosquitoes I hypothesized that they also induce mosquito aggregation in swarms and tune the mosquito auditory sensitivity to the flight tone of mating partners to maximize mating success. I argue that interfering with serotonin and octopamine signalling pathways could be a novel mosquito control target to impair hearing and swarming and consequently hinder mosquito mating. My preliminary data support this hypothesis: i) the levels of octopamine and serotonin receptors in the mosquito JO change during the time when mosquitoes are swarming, ii) the auditory sensitivity of male mosquito JO increases during swarming, and this effect is mimicked after injecting octopamine. In this project, I will use a multidisciplinary approach bridging the fields of molecular biology, physiology and behaviour to study the physiological and behavioural changes induced by octopamine and serotonin in malaria-transmitting Anopheles gambiae mosquitoes with the declared goal of devising novel vector control tools. To investigate this, I will first analyse the connection between the octopaminergic and serotonergic signalling in the mosquito JO and the circadian clock. I will study if octopamine and serotonin levels change during swarming and how these changes reflect shifts in auditory sensitivity. I will then disrupt these pathways by generating serotonin and octopamine receptor mutants and study the auditory and swarming behaviour phenotypes. Finally, I aim to translate these findings into innovative mosquito control tools by using the acquired knowledge to disrupt swarming behaviour in semi-field settings in a malaria endemic country.
蚊子比任何其他节肢动物对声音都更敏感,因为它们有迷人的微小触角耳朵--约翰斯顿器官(JO)。在比针头还小的器官中,雄性蚊子拥有大约15,000个听觉神经元,大致相当于人类耳蜗的毛发数量。蚊子用它们复杂的JO来识别交配伙伴的飞行音调。每天日落时分,雄性蚊子聚集在一起吸引雌性蚊子。在蜂群中,雄性和雌性通过翅膀的拍打声来探测对方。由于蚊子交配的必要性,交配前的声学相互作用和群集行为是蚊子控制工具的主要目标。尽管如此,蚊子群集行为的神经生物学,以及诱导它的生理机制,几乎是完全未知的。我们最近已经表明,蚊子JO功能是如何调制的听觉传出系统。这是值得注意的,因为听觉传出控制被认为只存在于脊椎动物中。我们表明,生物胺章鱼胺和血清素在蚊子JO中释放,并调节声音诱导的机械和电反应,这可能有助于JO作为声音探测器的复杂性。有趣的是,生物胺在其他昆虫中诱导群集和社会行为。综上所述,考虑到蚊子的听觉和群集行为之间的密切关系,我假设它们也会诱导蚊子群集,并调整蚊子对交配伴侣飞行音调的听觉敏感度,以最大限度地提高交配成功率。我认为,干扰血清素和章鱼胺信号通路可能是一种新的蚊子控制目标,损害听力和群集,从而阻碍蚊子交配。我的初步数据支持这一假设:i)在蚊子成群的时候,蚊子JO中的章鱼胺和5-羟色胺受体的水平会发生变化,ii)雄性蚊子JO的听觉灵敏度在成群的时候会增加,这种效果在注射章鱼胺后会被模仿。在这个项目中,我将使用一个多学科的方法桥接分子生物学,生理学和行为学领域的研究章鱼胺和血清素引起的生理和行为变化疟疾传播冈比亚按蚊蚊子与设计新的载体控制工具的宣布目标。为了研究这一点,我将首先分析在蚊子JO和昼夜节律钟的章鱼胺能和多巴胺能信号之间的连接。我将研究章鱼胺和5-羟色胺的水平是否会在群集过程中发生变化,以及这些变化如何反映听觉灵敏度的变化。然后,我将通过产生5-羟色胺和章鱼胺受体突变体来破坏这些通路,并研究听觉和群集行为表型。最后,我的目标是将这些发现转化为创新的蚊子控制工具,利用获得的知识来破坏疟疾流行国家半野外环境中的群集行为。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hitting the right note at the right time: Circadian control of audibility in Anopheles mosquito mating swarms is mediated by flight tones.
- DOI:10.1126/sciadv.abl4844
- 发表时间:2022-01-14
- 期刊:
- 影响因子:13.6
- 作者:Somers J;Georgiades M;Su MP;Bagi J;Andrés M;Alampounti A;Mills G;Ntabaliba W;Moore SJ;Spaccapelo R;Albert JT
- 通讯作者:Albert JT
Acoustic Physiology in Mosquitoes
蚊子的声学生理学
- DOI:10.1101/pdb.top107685
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Su M
- 通讯作者:Su M
Hearing of malaria mosquitoes is modulated by a beta-adrenergic-like octopamine receptor which serves as insecticide target.
- DOI:10.1038/s41467-023-40029-y
- 发表时间:2023-07-19
- 期刊:
- 影响因子:16.6
- 作者:Georgiades, Marcos;Alampounti, Alexandros;Somers, Jason;Su, Matthew P. P.;Ellis, David A.;Bagi, Judit;Terrazas-Duque, Daniela;Tytheridge, Scott;Ntabaliba, Watson;Moore, Sarah;Albert, Joerg T.;Andres, Marta
- 通讯作者:Andres, Marta
A novel beta-adrenergic like octopamine receptor modulates the audition of malaria mosquitoes and serves as insecticide target
- DOI:10.1101/2022.08.02.502538
- 发表时间:2022-08
- 期刊:
- 影响因子:0
- 作者:M. Georgiades;CA Alampounti;J. Somers;M. Su;D. Ellis;J. Bagi;W. Ntabaliba;S. Moore;JT Albert;M. Andrés
- 通讯作者:M. Georgiades;CA Alampounti;J. Somers;M. Su;D. Ellis;J. Bagi;W. Ntabaliba;S. Moore;JT Albert;M. Andrés
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Marta Andrés Miguel其他文献
Marta Andrés Miguel的其他文献
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{{ truncateString('Marta Andrés Miguel', 18)}}的其他基金
In the middle of the swarm: neuromodulation of the auditory function in malaria mosquitoes
在群体中间:疟疾蚊子听觉功能的神经调节
- 批准号:
MR/Y011732/1 - 财政年份:2024
- 资助金额:
$ 157.66万 - 项目类别:
Fellowship
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