ANALYSIS OF THE IMMUNE RESPONSE IN VISCERAL LEISHMANIASI

内脏利什曼原虫的免疫反应分析

• 批准号: 6547491
• 负责人: R T KENNEY
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6547491
• 关键词: Brazil; India; clinical research; human genetic material tag; human subject; immunity; immunomodulators; interleukin 12; leishmaniasis; polymerase chain reaction

Vaccination using defined antigens has been unsuccessful in leishmaniasis due to an inadequate understanding of the immune events leading to disease. We are pursing recent findings in the mouse model of leishmaniasis to investigate the cellular response to infection in humans both in vitro and in vivo. A clinical protocol is in place in Varanasi, India to study human bone morrow and splenic aspirates obtained during routine clinical evaluation of patients with visceral leishmaniasis. Dr. Kalpana Pai, an Immunobiologist in the Indian Laboratory visited the FDA Laboratory for 3 months to initiate other immune studies in the Indian population. The group in Vitoria, Brazil is looking at the potential for genetic factors to influence the immune response to infection evaluating polymorphisms in cytokine gene promoter regions in families with kala- azar. In the Laboratory here we have begun to look at the in vitro response to infection with promastigotes and amastigotes by evaluating the IFNgamma receptor activation pathway in macrophages and macrophage-like cells.

由于对导致疾病的免疫事件了解不足，使用确定抗原的疫苗接种在利什曼病中一直不成功。我们正在研究利什曼病小鼠模型的最新发现，以研究体外和体内人类对感染的细胞反应。印度瓦拉纳西有一项临床方案，用于研究内脏利什曼病患者常规临床评估中获得的人骨和脾脏抽吸物。印度实验室的免疫生物学家Kalpana Pai博士访问了FDA实验室3个月，以启动印度人群的其他免疫研究。巴西维多利亚的研究小组正在研究遗传因素影响感染免疫反应的可能性，评估黑热病家族中细胞因子基因启动子区域的多态性。在这里的实验室里，我们已经开始通过评估巨噬细胞和巨噬细胞样细胞中的ifnγ受体激活途径来观察对promastigotes和amastigotes感染的体外反应。