ANALYSIS OF THE IMMUNE RESPONSE IN VISCERAL LEISHMANIASIS

内脏利什曼病的免疫反应分析

• 批准号: 6161172
• 负责人: R T KENNEY
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161172
• 关键词: Brazil; India; clinical research; human genetic material tag; human subject; immunity; immunomodulators; interleukin 12; leishmaniasis; polymerase chain reaction

Vaccination using defined antigens has been unsuccessful in leishmaniasis due to an inadequate understanding of the immune events leading to disease. We are pursing recent findings in the mouse model of leishmaniasis to investigate the cellular response to infection in humans both in vitro and in vivo. A clinical protocol is in place in Varanasi, India to study human bone morrow and splenic aspirates obtained during routine clinical evaluation of patients with visceral leishmaniasis. Dr. Kalpana Pai, an Immunobiologist in the Indian Laboratory visited the FDA Laboratory for 3 months to initiate other immune studies in the Indian population. The group in Vitoria, Brazil is looking at the potential for genetic factors to influence the immune response to infection evaluating polymorphisms in cytokine gene promoter regions in families with kala- azar. In the Laboratory here we have begun to look at the in vitro response to infection with promastigotes and amastigotes by evaluating the IFNgamma receptor activation pathway in macrophages and macrophage-like cells.

使用确定抗原的疫苗接种在利什曼病中一直不成功 由于对免疫事件的不充分理解， 疾病我们正在研究最近在小鼠模型中的发现， 利什曼病研究人类感染的细胞反应 无论是在体外还是在体内。瓦拉纳西已经制定了一项临床方案， 印度将研究人类骨髓和脾穿刺液， 内脏利什曼病患者的常规临床评估。博士 印度实验室免疫生物学家Kalpana派访问FDA 实验室3个月，在印度启动其他免疫研究 人口巴西维托里亚的研究小组正在研究 影响感染免疫应答的遗传因素 Kala家族中细胞因子基因启动子区的多态性 阿扎在这里的实验室里，我们已经开始研究体外 通过评估前鞭毛体和无鞭毛体感染的反应， 巨噬细胞和巨噬细胞样细胞中IFN γ受体活化途径 细胞