CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 6268442
• 负责人: WILLIAM A HORTON
• 金额: $ 13.52万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268442
• 关键词: animal tissue; biomedical facility; electron microscopy; immunoelectron microscopy; laboratory mouse; scanning transmission electron microscopy; skeletal system; transmission electron microscopy

The electron microscopy (EM) core in Portland will carry out transmission EM and, when appropriate, scanning EM and immuno (gold) EM studies on the murine skeletal tissues and rotary shadowing of fibrillar constituents of the skeletal matrices. In some instances i.e., transmission EM, mouse skeletal tissues will be fixed in Houston and shipped to Portland. In others, i.e., immuno EM, mice will be transferred to Portland so that the tissues of interest can be exposed to antibody before fixation. All of these protocols are currently in operation in the facility, and a wide range of antibodies that recognize murine skeletal protein epitopes are available. The investigators, Dr. William Horton and Doug Keene, have a long standing interest and special expertise in this field, and the EM facility in Portland is particularly suited for the proposed studies. Moreover, both investigators have collaborated closely and productively with other PIs in the program project demonstrating how mice, mouse embryos, murine skeletal tissues and information can be easily transferred from one location to the other as well as the effectiveness of this working arrangement. The ultrastructural analyzes to be provided by this core are essential to the overall performance of the program project.

波特兰的电子显微镜(EM)核心将进行传输 在适当的情况下，扫描电子显微镜和免疫(金)电子显微镜研究 小鼠骨骼组织和纤维成分的旋转阴影 骨架矩阵。在一些情况下，即传输EM、鼠标 骨骼组织将在休斯顿固定，然后运往波特兰。在……里面 其他的，即免疫EM小鼠将被转移到波特兰，以便 在固定前，可将感兴趣的组织暴露于抗体。所有的 这些协议目前在该设施中运行，并有广泛的 识别小鼠骨骼蛋白表位的抗体范围包括 可用。 调查人员威廉·霍顿博士和道格·基恩长期以来 对这一领域的兴趣和专门知识，以及位于 波特兰特别适合进行拟议中的研究。此外，两者 调查人员与其他私家侦探密切合作，在 该项目展示了小鼠、小鼠胚胎、小鼠骨骼 组织和信息可以很容易地从一个位置传输到 以及这一工作安排的有效性。 由该岩芯提供的超微结构分析对于 计划项目的整体表现。