CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 6268442
• 负责人: WILLIAM A HORTON
• 金额: $ 13.52万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268442
• 关键词: animal tissue; biomedical facility; electron microscopy; immunoelectron microscopy; laboratory mouse; scanning transmission electron microscopy; skeletal system; transmission electron microscopy

The electron microscopy (EM) core in Portland will carry out transmission EM and, when appropriate, scanning EM and immuno (gold) EM studies on the murine skeletal tissues and rotary shadowing of fibrillar constituents of the skeletal matrices. In some instances i.e., transmission EM, mouse skeletal tissues will be fixed in Houston and shipped to Portland. In others, i.e., immuno EM, mice will be transferred to Portland so that the tissues of interest can be exposed to antibody before fixation. All of these protocols are currently in operation in the facility, and a wide range of antibodies that recognize murine skeletal protein epitopes are available. The investigators, Dr. William Horton and Doug Keene, have a long standing interest and special expertise in this field, and the EM facility in Portland is particularly suited for the proposed studies. Moreover, both investigators have collaborated closely and productively with other PIs in the program project demonstrating how mice, mouse embryos, murine skeletal tissues and information can be easily transferred from one location to the other as well as the effectiveness of this working arrangement. The ultrastructural analyzes to be provided by this core are essential to the overall performance of the program project.

波特兰的电子显微镜（EM）核心将进行传输 EM和扫描EM和免疫（金）EM研究（如适用） 小鼠骨骼组织和旋转阴影的纤维成分 骨骼基质 在某些情况下，即，透射电镜，小鼠 骨骼组织将在休斯顿固定，然后运往波特兰。 在 其他人，即，免疫EM，小鼠将被转移到波特兰， 感兴趣的组织可以在固定之前暴露于抗体。 所有 这些协议目前正在设施中运行， 一系列识别鼠骨骼蛋白表位的抗体， available. 调查人员威廉·霍顿博士和道格·基恩长期以来 在这一领域的兴趣和特殊专长，以及EM设施， 波特兰特别适合进行拟议的研究。 而且两 调查人员与其他PI密切合作， 该项目展示了小鼠、小鼠胚胎、小鼠骨骼 组织和信息可以很容易地从一个地方转移到 以及这一工作安排的有效性。 该岩心所提供的超微结构分析对于 计划项目的整体绩效。