CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 6235835
• 负责人: WILLIAM A HORTON
• 金额: $ 12.95万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235835
• 关键词: animal tissue; biomedical facility; electron microscopy; immunoelectron microscopy; laboratory mouse; scanning transmission electron microscopy; skeletal system; transmission electron microscopy

The electron microscopy (EM) core in Portland will carry out transmission EM and, when appropriate, scanning EM and immuno (gold) EM studies on the murine skeletal tissues and rotary shadowing of fibrillar constituents of the skeletal matrices. In some instances i.e., transmission EM, mouse skeletal tissues will be fixed in Houston and shipped to Portland. In others, i.e., immuno EM, mice will be transferred to Portland so that the tissues of interest can be exposed to antibody before fixation. All of these protocols are currently in operation in the facility, and a wide range of antibodies that recognize murine skeletal protein epitopes are available. The investigators, Dr. William Horton and Doug Keene, have a long standing interest and special expertise in this field, and the EM facility in Portland is particularly suited for the proposed studies. Moreover, both investigators have collaborated closely and productively with other PIs in the program project demonstrating how mice, mouse embryos, murine skeletal tissues and information can be easily transferred from one location to the other as well as the effectiveness of this working arrangement. The ultrastructural analyzes to be provided by this core are essential to the overall performance of the program project.

波特兰的电子显微镜（EM）核心将进行传输 EM，并在适当的情况下扫描EM和免疫（黄金）EM研究 鼠骨骼组织和原纤维成分的旋转阴影 骨骼矩阵。 在某些情况下，即传输EM，鼠标 骨骼组织将固定在休斯敦，并运往波特兰。 在 其他人，即免疫EM，小鼠将被转移到波特兰 感兴趣的组织可以在固定前暴露于抗体。 所有人 这些协议目前正在设施中运行，并且很广 识别鼠骨骼蛋白表位的一系列抗体是 可用的。 调查人员威廉·霍顿（William Horton）和道格·基恩（Doug Keene） 该领域的兴趣和特殊专业知识以及EM设施 波特兰特别适合拟议的研究。 而且，这两个 调查人员与其他PI紧密合作 该程序项目展示了小鼠，鼠标，鼠骨骼如何 组织和信息可以很容易地从一个位置转移到 其他以及这种工作安排的有效性。 该核心提供的超微结构分析对于 计划项目的整体绩效。