GROWTH PLATE STUDIES IN THE CHONDRODYSTROPHIES
软骨营养不良的生长板研究
基本信息
- 批准号:3319058
- 负责人:
- 金额:$ 0.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1985
- 资助国家:美国
- 起止时间:1985-08-01 至 1990-12-31
- 项目状态:已结题
- 来源:
- 关键词:autoradiography bone development disorder cartilage development cartilage disorder cartilage metabolism cell differentiation cell growth regulation chondrocytes chondrodystrophy chondroitin chondroitin sulfates collagen connective tissue disorder connective tissue metabolism electron microscopy extracellular matrix fibronectins gel electrophoresis high performance liquid chromatography histochemistry /cytochemistry hyaluronate image processing immunochemistry in situ hybridization keratan sulfate microscopy monoclonal antibody normal ossification nucleic acid probes organ culture procollagen protein biosynthesis proteoglycan radiotracer scintillation counter tritium
项目摘要
The chondrodystrophies (CDX) are a heterogeneous group of disorders of
endochondral bone growth that are characterized clinically by shortening
and deformities of the limbs and trunk. The delineation of the
pathogenetic mechanisms responsible for the disorders would be highly
desirable because not only would it lead to definitive therapy for the CDX,
but it would provide valuable insights into the causes of skeletal
deformities in general as well as normal bone growth. Unfortunately, the
understanding of the abnormalities in the CDX has been hampered by a
variety of problems primarily related to obtaining and investigating the
affected tissue, the skeletal growth plate. Addressing these problems,
the proposed investigator has developed an approach tailored to studying
these disorders. It involves the use of highly specific histochemistry to
localize matrix constituents in plastic embedded tissues combined with
biochemical analysis of extremely small samples of cartilage. Preliminary
studies have demonstrated its potential value as a means to probe the
growth plate as a dynamic structure. It is now proposed to expand this
approach to systematically and comprehensively examine endochondral
ossification in growth plate tissue from normal control and CDX patients.
The tissue will be studied by light and electron microscopic immune and
lectin histochemistry, autoradiography, and in situ hybridization.
Monoclonal antibodies to matrix components: types I-VI, IX (G), X (M), and
1Alpha, 2Alpha, 3Alpha collagens; chondroitin-4- and -6- sulfate, keratan
sulfate and hyaluronic acid binding region substructures of cartilage
proteoglycan; link protein; fibronectin; and cDNA probes to specific
skeletal procollagen mRNAs will be employed. To biochemically confirm the
microscopic observations, microsamples of the cartilages corresponding to
different stages of chondrocyte proliferation and differentiation will be
studied in organ culture by HPLC and SDS-PAGE methods to determine the
presence of and relative proportions of isotopically labeled major and
minor collagens and glycosaminoglycans in newly synthesized matrix. These
studies should permit an accurate representation of the events of normal
endochondral ossification to be constructed and defects therein to be
identified.
软骨营养不良(CDX)是一组不同类型的疾病
临床上以缩短为特征的软骨内骨生长
四肢和躯干的畸形。世界上最大规模的
导致这些疾病的致病机制很可能是
可取的,因为它不仅会导致对CDX的最终治疗,
但它将对骨骼的原因提供有价值的见解
全身畸形以及正常的骨骼生长。不幸的是,
对CDX异常的理解受到了一种
主要与获取和调查
受影响的组织,骨骼生长板。解决这些问题,
拟议的调查员已经制定了一种适合研究的方法
这些障碍。它涉及到使用高度特异的组织化学来
局部定位塑料包埋组织中的基质成分
对极少量的软骨样本进行生化分析。初步
研究表明,它作为一种手段来探索
生长板作为一种动态结构。现在建议扩大这一范围
一种系统全面检查软骨内的方法
正常对照组和CDX患者生长板组织中的骨化。
组织将通过光学和电子显微镜免疫和
凝集素组织化学、放射自显影和原位杂交。
抗基质成分的单抗:I-VI、IX(G)、X(M)和
1α,2α,3α胶原蛋白;软骨素-4-和-6-硫酸盐,角质形成
软骨的硫酸盐和透明质酸结合区亚结构
蛋白多糖;连接蛋白;纤维连接蛋白;以及特异的
将使用骨骼前胶原mRNAs。以生化方法确认
显微镜观察,软骨的微量样本对应于
软骨细胞增殖分化的不同阶段
用高效液相色谱和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对器官培养进行了研究
同位素标记的主要和相对比例的存在和
新合成基质中的少量胶原蛋白和糖胺聚糖。这些
研究应该允许对正常事件的准确呈现
待构建的软骨内骨化及其缺损区
确认身份。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WILLIAM A HORTON其他文献
WILLIAM A HORTON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WILLIAM A HORTON', 18)}}的其他基金
Urinary biomarkers to assess linear bone growth velocity
用于评估线性骨生长速度的尿液生物标志物
- 批准号:
8621082 - 财政年份:2013
- 资助金额:
$ 0.5万 - 项目类别:
Urinary biomarkers to assess linear bone growth velocity
用于评估线性骨生长速度的尿液生物标志物
- 批准号:
8737013 - 财政年份:2013
- 资助金额:
$ 0.5万 - 项目类别:














{{item.name}}会员




