Quantitation of Biomarkers of Oxidative Stress by NICI-MS

通过 NICI-MS 定量氧化应激生物标志物

• 批准号: 6106737
• 负责人: Kenneth Tomer
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6106737
• 关键词: alcoholic beverage consumption; antioxidants; biomarker; carcinogenesis; carotenoids; clinical research; eicosanoids; human data; human tissue; lipid peroxides; mass spectrometry; nutrition aspect of cancer; nutrition related tag; oxidative stress; peroxidation; tissue /cell culture

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. The mass spectrometry workgroup has agreed to undertake these analyses within NIEHS. We have since elaborated the analytical scheme to improve overall yield and reliability of the sample workup protocol, and are currently determining isoprostane levels in the round-robin study. A number of other studies involving measurement of isoprostanes as an indicator of oxidative stress have also been initiated. One such study is a collaboration with NCI (Dr. Phil Taylor) and USDA (Drs. David Baer and Joseph Judd) as part of the Women's Alcohol Study. The aim of the part of the study we are participating in is to determine the effect of alcohol consumption on lipid levels, oxidative stress, and micronutrient levels in postmenopausal women, and to assess the influence of alcohol and hormone metabolism genetic polymorphism on hormone levels. A second study probes questions raised by recent prevention and intervention trials that implied that consumption of carotenoid-rich foods early in disease states provides a protective advantage while late intervention with dietary carotenoids exacerbates cancer. It is hypothesized that an anti- oxidant environment only during the earliest stage of tumorigenesis will ultimately decrease the incidence and severity of tumors; conversely, the same anti-oxidant regimens fed after appearance of preneoplastic foci will lead to exacerbation of the subsequent tumor burden. We will provide quantitative measurement of oxidative stress in these studies which include both animal and cell culture studies.

1996年氧化测量研讨会 人类压力在 NIEHS 举办。曾有一位将军 一致认为开展一项比较研究是有价值的 从同一样品中测量的氧化应激的各种标记物。 其中一种标记物是 F2a-异前列烷，其定量通过 NICI-MS。质谱工作组已同意 在 NIEHS 内进行这些分析。 此后我们详细阐述了 提高整体产量和可靠性的分析方案 示例后处理方案，目前正在测定异前列烷 循环研究中的水平。许多其他研究涉及 测量异前列腺素作为氧化应激指标 也已启动。其中一项研究是与 NCI 合作进行的 （菲尔·泰勒博士）和美国农业部（大卫·贝尔博士和约瑟夫·贾德博士） 女性酒精研究的一部分。该部分的目的 我们正在参与的研究是为了确定酒精的影响 消耗对脂质水平、氧化应激和微量营养素的影响 绝经后妇女的水平，并评估其影响 酒精与激素代谢的基因多态性 激素水平。第二项研究探讨了最近提出的问题 预防和干预试验表明消费 疾病早期富含类胡萝卜素的食物可提供保护作用 膳食类胡萝卜素后期干预的优势 加剧癌症。据推测，一种抗氧化剂 只有在肿瘤发生的最早阶段，环境才会 最终降低肿瘤的发生率和严重程度； 相反，出现相同的抗氧化方案后 癌前病灶会导致随后肿瘤的恶化 负担。我们将提供氧化的定量测量 这些研究中的压力，包括动物和细胞培养 研究。