Quantitation of Biomarkers of Oxidative Stress by NICI-MS

1996年，举办了一次关于氧化性测量的研讨会， 人类的压力在NIEHS举行。人们普遍 一致认为，开展一项研究， 从同一样品测量的氧化应激的各种标志物。 一种这样的标志物是F2 a-异前列烷，其通过以下方法定量： NICI-MS。质谱分析师已经同意 在NIEHS中进行这些分析。 此后，我们详细阐述了 提出了提高整体成品率和可靠性的分析方案， 样本处理方案，目前正在测定异前列烷 在循环研究中。其他一些研究， 作为氧化应激指标的异前列烷的测量 也已经启动。其中一项研究是与NCI的合作 (Dr.菲尔·泰勒）和美国农业部（大卫贝尔和约瑟夫·贾德博士）作为 女性酒精研究的一部分本部分的目的是 我们正在参与的一项研究是确定酒精的影响， 消耗对脂质水平、氧化应激和微量营养素 水平，并评估影响 酒精和激素代谢遗传多态性 荷尔蒙水平第二项研究探讨了最近几年 预防和干预试验表明， 在疾病早期富含类胡萝卜素的食物提供了一种保护性的 优势，而后期干预与膳食类胡萝卜素 会加剧癌症据推测，抗氧化剂 只有在肿瘤发生的最早阶段， 最终降低肿瘤的发生率和严重程度; 相反，在出现以下情况后喂食相同的抗氧化剂方案 癌前病灶将导致随后的肿瘤恶化 负担我们将提供定量测量氧化 这些研究包括动物和细胞培养 问题研究

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. The mass spectrometry workgroup has agreed to undertake these analyses within NIEHS. We have since elaborated the analytical scheme to improve overall yield and reliability of the sample workup protocol, and are currently determining isoprostane levels in the round-robin study. A number of other studies involving measurement of isoprostanes as an indicator of oxidative stress have also been initiated. One such study is a collaboration with NCI (Dr. Phil Taylor) and USDA (Drs. David Baer and Joseph Judd) as part of the Women's Alcohol Study. The aim of the part of the study we are participating in is to determine the effect of alcohol consumption on lipid levels, oxidative stress, and micronutrient levels in postmenopausal women, and to assess the influence of alcohol and hormone metabolism genetic polymorphism on hormone levels. A second study probes questions raised by recent prevention and intervention trials that implied that consumption of carotenoid-rich foods early in disease states provides a protective advantage while late intervention with dietary carotenoids exacerbates cancer. It is hypothesized that an anti- oxidant environment only during the earliest stage of tumorigenesis will ultimately decrease the incidence and severity of tumors; conversely, the same anti-oxidant regimens fed after appearance of preneoplastic foci will lead to exacerbation of the subsequent tumor burden. We will provide quantitative measurement of oxidative stress in these studies which include both animal and cell culture studies.