CORE FACILITY FOR THE VISUAL SCIENCES

28名首席调查人员，在 凯斯西储大学，克利夫兰大学医院， 克利夫兰退伍军人管理局医疗中心 组成了由眼科组成的核心小组， CWRU的遗传学、神经学、神经科学和病理学 发展多学科方法来研究主要研究领域 视觉科学方面：CWRU视觉科学研究中心。 拟议的NEI核心基金将促进以下各方的合作 这组调查人员包括九名NEI资助的校长 调查人员和9名提交待决或计划提交的材料。这个 核心基金将由三个研究支助模块组成：1. 组织培养和细胞分析模块将协助调查人员 为建立和维持各种细胞系，提供组织 为组织学、电子显微镜和 免疫细胞化学，并提供最先进的设施 用于流式细胞术、细胞分类和共聚焦显微镜。2. 分子生物学模块将提供熟练的技术专业知识， 克隆和扩增DNA测序的设备和技术， DNA和RNA的聚合酶链式反应扩增和分析、定量和 DNA凝胶的数字成像、细菌转化和表达 蛋白质，以及常用细菌菌株、载体、 和cDNAs构建。3.特色动物资源 模块将提供技术和搬运支持、隔离架 和笼子，以及专门为生产子代而寄生的 与眼睛研究相关的小鼠和大鼠品系。

Twenty-eight principal investigators, conducting vision research at Case Western Reserve University, University Hospitals of Cleveland, and the Cleveland Veterans Administration Medical Center have constituted a core group from the Departments of Ophthalmology, Genetics, Neurology, Neurosciences, and pathology at CWRU to develop multidisciplinary approach to examine major areas of study in the visual sciences: the CWRU Visual Sciences Research Center. The proposed NEI Core Facility will facilitate collaboration among this group of investigators that includes nine NEI-funded principal investigators and nine with pending or planned submissions. The Core Facility will be composed of three research support modules: 1. The Tissue Culture and Cell Analysis Module will assist investigators to establish and maintain a variety of cell lines, provide tissue preparation for histology, electron microscopy, and immunocytochemistry, and offer access to state-of-the-art facilities for flow cytometry, cell sorting, and confocal microscopy. 2. The Molecular Biology Module will provide skilled technical expertise, equipment and techniques for sequencing clone and amplified DNA, PCR amplification and analysis of DNA and RNA, quantitation and digital imaging of DNA gels, bacterial transfection and expression of proteins, and storage of commonly used bacterial strains, vectors, and cDNA constructs. 3. The Specialized Animal Resources Module will provide technical and handling support, isolation racks and cages, and boarding for the production of progeny of specialized mouse and rat strains that are relevant to studies of the eyes.