METAL ION COMPLEXATION

金属离子络合

• 批准号: 6271528
• 负责人: ANTHONY FRATIELLO
• 金额: $ 13.37万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271528
• 关键词: anions; bioenergetics; cations; chemical structure function; heterocyclic compounds; lanthanum; ligands; metal complex; nitrogen compounds; nuclear magnetic resonance spectroscopy; superconductivity

The objectives of this research are the quantitative determination of the coordination and complexing properties of the trivalent lanthanide cations, and the subsequent application of this information to the study of these ions with ligands of physiological importance. The lanthanides are used extensively as binding agents for interaction site determinations of biological ligands, as probe substituents in metalloenzymes and other systems requiring a metal-ion for activity, as relaxation agents in magnetic resonance imaging, as NMR chemical shift reagents, and as components in numerous industrial processes. The information sought by the proposed experiments includes the maximum coordination number at our conditions of measurement; the determination of variations, if any, of this value through the lanthanide series; the presence of inner-shell complexing and its dependence on anion and solvent properties; the extent of competitive solvation and its dependence on ligand properties; and in all cases, the quantitative determination of the structures of the species in solution. This information will provide a solid framework for the coordination study of four principal deoxyribonucleotides and their component molecules as an initial group of ligands. The experimental techniques to be used permit the direct observation of NMR signals for the components of the primary cation solvation shell, specifically solvent ligands, an ions, and in some systems, the diamagnetic metal-ion itself. In turn, these observations lead to the quantitative evaluation of the lanthanide coordination properties mentioned above. The measurements will be made using a Bruker AM-400 multinuclear, superconducting, Fourier transform NMR spectrometer. Depending on the salt and solvent system, the chemical shift, area, and linewidth techniques to be applied will involve the 1H, 13C, 15N, 31P, 35Cl, 81Br, 127I, and diamagnetic metal-ion nuclei where possible.

本研究的目的是定量测定 三价镧系元素的配位和络合特性 阳离子，以及随后将此信息应用于研究 这些离子与具有生理重要性的配体的结合。镧系元素 广泛用作相互作用位点测定的结合剂 生物配体，作为金属酶和其他酶中的探针取代基 需要金属离子进行活性的系统，作为松弛剂 磁共振成像，作为 NMR 化学位移试剂，以及 许多工业过程中的组件。所寻求的信息 提议的实验包括我们的最大配位数 测量条件；确定变化（如果有的话） 该值通过镧系元素；内壳的存在 络合及其对阴离子和溶剂性质的依赖性；程度 竞争性溶剂化及其对配体性质的依赖性；并在 所有情况下，物种结构的定量测定 在溶液中。这些信息将为 四种主要脱氧核糖核苷酸及其配位研究 组分分子作为初始配体基团。 所使用的实验技术允许直接观察 主要阳离子溶剂化壳层组分的 NMR 信号， 特别是溶剂配体、离子，并且在某些系统中， 抗磁性金属离子本身。反过来，这些观察导致 镧系元素配位特性的定量评价 上面提到过。测量将使用 Bruker AM-400 进行 多核、超导、傅里叶变换核磁共振波谱仪。 根据盐和溶剂系统，化学位移、面积和 所应用的线宽技术将涉及1H、13C、15N、31P、 35Cl、81Br、127I 和反磁性金属离子核（如果可能）。