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ACQUISITION OF A 400 MHZ NMR SPECTROMETER FOR RESEARCH

购买 400 MHZ 核磁共振波谱仪用于研究

基本信息

批准号：
2503787
负责人：
BRUCE GANEM
金额：
$ 33万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-05-20 至 1999-05-19
项目状态：
已结题

项目摘要

This proposal is a request for the funds required to purchase a 400 MHz NMR spectrometer to expand and update current capabilities in NMR instrumentation at Cornell University. The requested spectrometer will be devoted to structural studies of small molecules in liquids and will provide modern techniques to a core group of NIH-supported users. The spectrometer will be housed in the Chemistry Department NMR Facility, which provides instrumentation and training to almost 200 students and researchers from the university community and local industry. Five NIH- funded Cornell faculty members require access to this spectrometer to carry out a variety of health-related projects. Professor B. Ganem (Principal Investigator) will study (1) potential inhibitors of chorismate mutase/prephenate dehydratase involved in the shikimic acid pathway, (2) the synthesis of glycosidase inhibitors, (3) the synthesis of purine nucleoside phosphorylase inhibitors important for the treatment of AIDS, and (4) inhibitors of glutathione and trypanothione reductases. Professor T. Begley will investigate enzyme function and mechanism by using NMR spectroscopy to elucidate the structure of products of enzyme-catalyzed reactions. These reactions include the (6) thiamin biosythetic pathway, (7) enzymatic repair of UV-irradiated DNA in bacterial spores, (8) the mechanism and inhibition of prolyl-4- hydroxylase, and (9) the biosynthesis of taxol. Professor J. Clardy will investigate natural products from novel sources including (10) anticancer compounds isolated from endophytic fungi and (11) compounds displaying antibiotic properties toward the plant pathogens responsible for fire blight. Professor D. B. Collum will investigate (5) organolithium solvation and aggregation using 6 Li-detected triple- resonance spectroscopy. Professor J. Meinwald will continue to pioneer the field of chemical ecology by exploring (12) biologically active compounds isolated from insect sources.

这份提案是对购买400兆赫所需资金的请求核磁共振波谱仪将扩展和更新目前的核磁共振功能康奈尔大学的仪器。所请求的分光计将致力于液体和意志中小分子的结构研究为NIH支持的核心用户群体提供现代技术。这个光谱仪将被安置在化学系核磁共振设施中，它为近200名学生提供仪器和培训，来自大学社区和当地产业界的研究人员。五个美国国立卫生研究院- 康奈尔大学资助的教职员工需要使用这台光谱仪开展多种健康相关项目。B·加纳姆教授 (首席调查员)将研究(1)潜在的抑制剂分支酸变位酶/预苯酸脱水酶与莽草酸的关系途径，(2)糖苷酶抑制剂的合成，(3)合成嘌呤核苷磷酸化酶抑制剂对艾滋病的治疗，以及(4)谷胱甘肽和色氨酸的抑制剂还原酶。T.Begley教授将研究酶的功能和用核磁共振谱阐明其结构的作用机理酶催化反应的产物。这些反应包括(6) 硫胺素生物合成途径，(7)紫外线照射下DNA的酶修复在细菌孢子中，(8)Pro-4-的机理和抑制作用羟基酶；(9)紫杉醇的生物合成。J.克拉迪教授将研究来自新来源的天然产品，包括(10) 从内生真菌中分离的抗癌化合物和(11)个化合物对负责的植物病原菌展示抗生素特性治疗火疫病。D·B·科勒姆教授将进行调查(5) 有机锂的溶剂化和聚集化共振光谱学。J.Meinwald教授将继续开创探索具有生物活性的化学生态领域(12) 从昆虫中分离出来的化合物。