CORE--STRUCTURAL DATA FROM NMR SPECTROSCOPY

核心——核磁共振波谱的结构数据

• 批准号: 6107890
• 负责人: JOHN ORBAN
• 金额: $ 15.24万
• 依托单位: UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6107890
• 关键词: Haemophilus; bacterial proteins; biomedical facility; data collection; nuclear magnetic resonance spectroscopy; protein folding; protein structure

DESCRIPTION: This core project will provide NMR data on some members of the set of expressed H. influenzae proteins that will be provided by other components of the project. NMR will be used in early stages to screen conditions for solubility (in states of low aggregation), and the degree of folding of the protein. When suitable conditions are found, labeled protein will be generated and resonance assignments will be determined using multi-nuclear multi-dimensional methods. With backbone assignments, a quick assessment of secondary structure can be made, which will feed into the structure prediction effort. Further analysis of NMR data will then lead to identification of the fold (a low resolution structure), and can be pushed on to give high resolution structure when desired. The effort will be guided by Dr. Organ, and largely executed by one Research Associate (Dr. Sari), with about 1/2 of a 600 MHz spectrometer dedicated to the work. The expected culprit will be the order of 2 low resolution and 1 high-resolution structure per year.

描述：该核心项目将提供一些成员的NMR数据， 表示的H.流感病毒蛋白质，将提供其他 项目的组成部分。核磁共振将在早期阶段用于筛选 溶解度的条件（在低聚集状态下），以及 蛋白质的折叠。当找到合适的条件时， 将产生蛋白质并确定共振分配 使用多核多维方法。有了骨干分配， 可以对二级结构进行快速评估， 投入到结构预测工作中。核磁共振数据的进一步分析将 然后导致褶皱的识别（低分辨率结构），并且 当需要时，可以被推上去以给出高分辨率结构。的 这项工作将由Organ博士指导，主要由一个研究小组执行。 助理（Sari博士），约1/2的600 MHz频谱仪专用 去工作。预期的罪魁祸首将是2低分辨率的顺序 每年一个高分辨率的结构。