CORE--TISSUE ACQUISITION, PROCESSING AND ANALYSIS

组织采集、处理和分析核心是 扩大职责包括适当分配的病理科 和组织的加工，用于研究的常规诊断工作之外 人类以及对犬骨髓移植的解读 组织病理学。该核心管理收集、日志记录、存储、 生物组织标本及其分析的处理与分析 识别这笔赠款的数据和跟踪。该单位协调所有 组织标本相关数据的收集和分发到病理学和 弗雷德·哈钦森癌症研究中心(FHCRC)的其他研究实验室。 例如，骨髓被分成血液学标本， 病理学、细胞遗传学、细胞相互作用、流式细胞术、细胞因子 实验室，以及其他实验室。这些研究中约有一半是 不可收费的，而且在一小部分可收费的研究是无法收取的。 长期随访标本由经验丰富的独一无二的人看到 病理科工作人员的账单是否可以收集。处理包括 冷冻、固定、包埋、储存和适当分配给 校内FHCRC实验室的子集。可使用的分析方法 病理学包括研究免疫组织化学、原位杂交(AS Y染色体DNA)、聚合酶链式反应(PCR)、荧光检测 原位杂交(FISH)和特异性移植物抗宿主病(GVHD) 研究(例如)原位末端标记法(TUNEL法)检测人和犬骨髓移植 收件人。自动化新方法论的研究与开发 免疫组织化学、原位杂交和聚合酶链式反应提供“前沿” 支持研究项目研究的技术 计划项目。

The Tissue Acquisition, Processing and Analysis Core is an outgrowth of the pathology section whose expanded responsibilities include proper allocation and processing of tissue for research beyond the routine diagnostic work in humans as well as interpretation of canine bone marrow transplant histopathology. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. The unit coordinates all tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, cellular interactions, flow cytometry, cytokine laboratories, and other laboratories. About half of these studies are nonbillable and in a fraction the billable studies are uncollectable. Long-term follow-up specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to subset of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft-versus-host disease (GVHD) studies (e.g.) Tunel stain for apoptosis) in human and canine marrow graft recipients. Research and development of new methodology in automated immunohistochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project.