CORE--TISSUE ACQUISITION, PROCESSING AND ANALYSIS

核心——组织采集、处理和分析

• 批准号: 6784821
• 负责人: George E Sale
• 金额: $ 10.42万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784821
• 关键词: animal tissue; biomedical facility; bone marrow; genetic techniques; histopathology; human tissue; immunocytochemistry; tissue /cell preparation; tissue resource /registry

The Tissue Acquisition, Processing and Analysis Core is an outgrowth of the pathology section whose expanded responsibilities include proper allocation and processing of tissue for research beyond the routine diagnostic work in humans as well as interpretation of canine bone marrow transplant histopathology. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. A particular new emphasis will be improved specimen tracking and specimen management, especially for the myelodysplasia project (Project 4) and cellular tracking and specimen management, especially for the myelodysplasia project (Project 4) and cellular interactions and marrow function project (Project 5). The unit coordinates coll tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, cellular interactions, flow cytometry, and other laboratories. About half of these studies are non-billable and in fraction the billable studies are uncollected. Long-term follow-specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to subsets of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft-versus-host disease (GVHD) diagnostic in human and canine marrow graft recipients. Research and development of new methodology in automated immunochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project.

组织采集、处理和分析核心是病理学部分的产物，其扩大的职责包括适当分配和处理组织，用于人类常规诊断工作以外的研究以及犬骨髓移植组织病理学的解释。该核心管理生物组织标本的收集、记录、储存、处理和分析及其识别数据和跟踪。一个特别的新重点将是改善标本跟踪和标本管理，特别是骨髓增生异常项目（项目4）和细胞跟踪和标本管理，特别是骨髓增生异常项目（项目4）和细胞相互作用和骨髓功能项目（项目5）。该单位协调科尔组织标本相关数据的收集和分发到病理学和其他弗雷德哈钦森癌症研究中心（FHCRC）研究实验室。例如，骨髓被分为血液学、病理学、细胞遗传学、细胞相互作用、流式细胞术和其他实验室的标本。这些研究中约有一半是不收费的，部分可收费的研究未收集。长期随访标本由经验丰富的病理学工作人员观察，无论账单是否可收取。处理包括冷冻、固定、包埋、储存和适当分发到FHCRC实验室内部的子集。病理学中可用的分析方法包括研究免疫组织化学、原位杂交（如Y染色体DNA）、聚合酶链反应（PCR）、荧光原位杂交（FISH）和人类和犬骨髓移植受者的专门移植物抗宿主病（GVHD）诊断。自动免疫化学，原位杂交和PCR新方法的研究和开发提供了“尖端”技术，以支持该计划项目内的研究项目的研究。