CORE A--TISSUE ACQUISITION, PROCESSING AND ANALYSIS

组织采集、处理和分析核心是病理科的衍生部门，其扩大的职责包括适当分配和处理组织，用于人类常规诊断工作之外的研究，以及对犬骨髓移植组织病理学的解释。该核心负责收集、记录、储存、处理和分析生物组织标本及其识别数据，并为这笔赠款进行跟踪。一个特别新的重点将是改进标本跟踪和标本管理，特别是骨髓发育不良项目(项目4)和细胞跟踪和标本管理，特别是骨髓发育不良项目(项目4)和细胞相互作用和骨髓功能项目(项目5)。该单位协调COIL组织标本相关数据的收集和分发到病理学和其他弗雷德·哈钦森癌症研究中心(FHCRC)的研究实验室。例如，骨髓被分成血液学、病理学、细胞遗传学、细胞相互作用、流式细胞术和其他实验室的标本。这些研究中约有一半是非收费的，有一小部分是收费的研究没有收集。长期随访标本由经验丰富的独特病理工作人员查看，无论账单是否可收集。加工包括冷冻、固定、埋藏、储存和适当地分配给FHCRC内部实验室的子集。病理学中可用的分析方法包括研究免疫组织化学、原位杂交(如Y染色体DNA)、聚合酶链式反应(PCR)、荧光原位杂交(FISH)和专门诊断人类和犬骨髓移植受者的移植物抗宿主病(GVHD)。自动化免疫化学、原位杂交和聚合酶链式反应新方法学的研究和开发提供了“尖端”技术，以支持计划项目中研究项目的研究。

The Tissue Acquisition, Processing and Analysis Core is an outgrowth of the pathology section whose expanded responsibilities include proper allocation and processing of tissue for research beyond the routine diagnostic work in humans as well as interpretation of canine bone marrow transplant histopathology. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. A particular new emphasis will be improved specimen tracking and specimen management, especially for the myelodysplasia project (Project 4) and cellular tracking and specimen management, especially for the myelodysplasia project (Project 4) and cellular interactions and marrow function project (Project 5). The unit coordinates coll tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, cellular interactions, flow cytometry, and other laboratories. About half of these studies are non-billable and in fraction the billable studies are uncollected. Long-term follow-specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to subsets of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft-versus-host disease (GVHD) diagnostic in human and canine marrow graft recipients. Research and development of new methodology in automated immunochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project.