CORE--PATHOLOGY

核心--病理学

• 批准号: 6300139
• 负责人: George E Sale
• 金额: $ 34.64万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-26 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300139
• 关键词: apoptosis; biomedical facility; biopsy; bone marrow exam; bone marrow transplantation; communicable disease diagnosis; data collection; diagnosis service; dogs; graft versus host disease; histopathology; human subject; immunocytochemistry; immunofluorescence technique; immunopathology diagnosis; in situ hybridization; leukemia; pathology; polymerase chain reaction; rapid diagnosis; tissue /cell culture; tissue /cell preparation

The pathology core, consisting of four pathology faculty and a staff of assistants, serves all of the program projects by providing anatomic pathology data collection and extensive professional anatomic pathology and other diagnostic consultative analysis. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. The unit coordinates all tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, flow cytometry, and other laboratories. Long-term follow-up specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to a subset of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft- versus-host disease (GVHD) studies (e.g., Tunel stain for apoptosis) in human and canine marrow graft recipients. Research and development of new methodology in automated immunohistochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project. Research in the pathogenesis and diagnosis of the diseases of bone marrow transplantation, such as graft-versus-host disease, venocclusive disease and other toxicity, infectious disease, and molecular diagnostics, also support the program's individual projects and the program as a whole.

病理学核心，包括四个病理学教师和工作人员 助理，通过提供服务，为所有的计划项目 解剖病理学数据收集和广泛的专业解剖 病理学和其他诊断咨询分析。 这一核心 管理收集、记录、存储、处理和分析 生物组织标本及其识别数据和跟踪， 这个grant。 该单位协调所有组织标本相关数据 收集和分发给病理学和其他弗雷德哈钦森 癌症研究中心（FHCRC）研究实验室。 骨髓， 例如被分为血液学，病理学， 细胞遗传学、流式细胞术和其他实验室。 长期 随访标本由经验丰富的病理学家观察， 工作人员是否收取帐单。 处理包括 冷冻、固定、包埋、储存和适当分配， FHCRC内部实验室的子集。 可用的分析方法 病理学包括研究免疫组织化学，原位 杂交（如Y染色体DNA）、聚合酶链反应 (PCR)，荧光原位杂交（FISH），和专门的移植- 抗宿主病（GVHD）研究（例如，凋亡的Tunel染色） 在人类和犬类骨髓移植接受者中。 研究和 自动化免疫组织化学新方法学的发展， 原位杂交和PCR提供了“尖端”技术， 支持计划内研究项目的研究 项目 疾病的发病机制和诊断研究 例如移植物抗宿主病， 静脉闭塞性疾病和其他毒性，传染病， 分子诊断，也支持该计划的个别项目 以及整个项目