CORE--METABOLISM AND FUNCTION

本核心将评估用于项目的正常和转基因小鼠模型中表面活性剂的代谢和表面活性剂的功能。本核心中使用的所有技术先前已在使用转基因小鼠的研究中发表。在此核心中使用的四种技术先前已在使用转基因小鼠的研究中发表。表面活性剂代谢和功能的四种测量方法将提供给项目：1。体内表面活性剂的代谢测量将包括a)肺泡灌洗液和肺组织中饱和磷脂酰胆碱（Sat PC）的池大小；B)选定研究的磷脂组成；c)放射性标记的前体掺入St PC和表面活性剂分泌动力学的测量；d)气管内注射放射性标记表面活性剂组分（Sat PC, SP-A, SP-B, SP-C）的清除动力学。这些测量提供了表面活性剂进出肺泡的通量信息。2. 表面活性剂的表面活性将在体外通过测量a)最小和最大表面张力来评估；b)表面活性剂的表面张力降低特性对血浆蛋白抑制的敏感性；c)吸收率。威廉天平和/或俘获泡表面计将用于上述测量。3. 将从小鼠肺泡叶中分离的表面活性剂给予缺乏表面活性剂的27 d妊娠早孕兔，在体内测定表面活性剂的功能。定量测定所试表面活性剂对早产兔肺通气动态顺应性和静压容积曲线的影响。

This core will assess surfactant metabolism a and surfactant function in the normal and transgenic mouse models used for the projects. All techniques to be used in this Core have been previously published in studies using transgenic mice. Four techniques to be used in this core have been previously published in studies using transgenic mice. Four types of measurements of surfactant metabolism and function will be provided to the projects: 1. Metabolic measurements of surfactant in vivo will include a) pool sizes of saturated phosphatidylcholine (Sat PC) in alveolar lavage and lung tissue; b) phospholipid composition for selected studies; c) radiolabeled precursor incorporation into St PC and measurements of the kinetics of surfactant secretion; and d) clearance kinetics for radiolabeled surfactant components (Sat PC, SP-A, SP-B, SP-C) given by intratracheal injection. These measurements provide information on the flux of surfactant into and out of the alveolus. 2. The surface activity of surfactant will be evaluated in vitro by measurements of a) minimum and maximum surface tension: b) sensitivity of surface tension lowering properties of surfactant to inhibition by plasma protein; and c) absorption rate. The Wilhelmy balance and/or captive bubble surfactometer will be used for above measurements. 3. Surfactant function will be measured in vivo by giving surfactant isolated from alveolar laves of mice to be surfactant deficient 27 d gestation premature rabbits. The effects of the test surfactants on dynamic compliance during ventilation and static pressure volume curves of the premature rabbit lung will be quantified.