CORE--METABOLISM AND FUNCTION

该核心将评估用于该项目的正常和转基因小鼠模型中的表面活性物质代谢a和表面活性物质功能。在这一核心中使用的所有技术都已在使用转基因小鼠的研究中发表。这一核心中将使用的四种技术此前已在使用转基因小鼠的研究中发表。将为项目提供四种类型的表面活性物质代谢和功能测量：1.表面活性物质在体内的代谢测量将包括a)肺泡灌洗液和肺组织中饱和磷脂酰胆碱(Sat PC)的池大小；b)选定研究的磷脂成分；c)放射性标记前体掺入ST PC并测量表面活性物质分泌动力学；d)气管内注射放射性标记表面活性物质组分(sat PC、SP-A、SP-B、SP-C)的清除动力学。这些测量提供了表面活性物质流入和流出肺泡的流量信息。2.表面活性剂的表面活性将通过测量a)最小和最大表面张力：b)表面张力降低特性对血浆蛋白抑制的敏感性和c)吸收率来评估。上述测量将使用Wilhelmy天平和/或捕获气泡表面测量仪。3.将从小鼠肺泡灌洗液中提取的肺表面活性物质给予肺表面活性物质缺乏性早产兔27天，在体内测定肺表面活性物质的功能。测试表面活性物质对早产兔肺的动态顺应性和静压容量曲线的影响将被量化。

This core will assess surfactant metabolism a and surfactant function in the normal and transgenic mouse models used for the projects. All techniques to be used in this Core have been previously published in studies using transgenic mice. Four techniques to be used in this core have been previously published in studies using transgenic mice. Four types of measurements of surfactant metabolism and function will be provided to the projects: 1. Metabolic measurements of surfactant in vivo will include a) pool sizes of saturated phosphatidylcholine (Sat PC) in alveolar lavage and lung tissue; b) phospholipid composition for selected studies; c) radiolabeled precursor incorporation into St PC and measurements of the kinetics of surfactant secretion; and d) clearance kinetics for radiolabeled surfactant components (Sat PC, SP-A, SP-B, SP-C) given by intratracheal injection. These measurements provide information on the flux of surfactant into and out of the alveolus. 2. The surface activity of surfactant will be evaluated in vitro by measurements of a) minimum and maximum surface tension: b) sensitivity of surface tension lowering properties of surfactant to inhibition by plasma protein; and c) absorption rate. The Wilhelmy balance and/or captive bubble surfactometer will be used for above measurements. 3. Surfactant function will be measured in vivo by giving surfactant isolated from alveolar laves of mice to be surfactant deficient 27 d gestation premature rabbits. The effects of the test surfactants on dynamic compliance during ventilation and static pressure volume curves of the premature rabbit lung will be quantified.