LUNG MATURATION

本研究项目对肺成熟将集中在一些 提供了动物肺成熟的功能和生化标志物， 早产儿兰姆中心的这个项目 这些动物将被 通过超声注射治疗胎儿与激素给予单 剂量和组合（皮质类固醇，T3，TRH和EGF） 未经处理的胎羊 本项目的目标是描述 通过评估出生后肺功能来评估肺反应， 通过连续测量气体交换、肺顺应性、肺 容量（40 cmH 2 O时的功能残气量和肺容量 压力）和对外源性表面活性剂的反应 早产后几小时。 随后，肺泡表面活性剂 饱和磷脂酰胆碱和SP-A池大小将测量为 将抗氧化酶水平和SP-A、SP-B和SP-C在mRNA水平 在肺组织和从组织中分离的II型细胞中。 这些 多个终点将反映功能和生化 早产羔羊诱导肺成熟反应的评估 暴露于短期临床相关激素治疗 战略布局

This Research Project on Lung Maturation will focus on a number of functional and biochemical markers of lung maturation in animals provided to this project by the Preterm Lamb Core. These animals will have been treated by ultrasound injections as fetuses with hormones given as single doses and as combinations (corticosteroid, T3, TRH and EGF) to previously unmanipulated fetal sheep. The goals of this project are to characterize the pulmonary responses by evaluating postnatal lung function as assessed by sequential measurements of gas exchange, lung compliances, lung volumes (functional residual capacity and lung volumes at 40 cmH2O pressures) and responses to exogenous surfactant over the first five hours after preterm delivery. Subsequently, alveolar surfactant saturated phosphatidylcholine and SP-A pool sizes will be measured as will antioxidant enzyme levels and SP-A, SP-B, and SP-C in mRNA levels in lung tissue and type II cells isolated from the tissue. These multiple endpoints will reflect both functional and biochemical assessments of the induced lung maturation responses in preterm lambs exposed to short duration, clinically relevant hormone treatment strategies.