CORE--METABOLISM AND FUNCTION

该核心将评估用于项目的正常和转基因小鼠模型中的表面活性剂代谢和表面活性剂功能。本核心中使用的所有技术先前已在使用转基因小鼠的研究中发表。四种技术将用于此 核心已经在使用转基因小鼠的研究中发表。四种类型的表面活性剂代谢和功能的测量将提供给项目：1。表面活性剂的体内代谢测量将包括a）肺泡灌洗液和肺组织中饱和磷脂酰胆碱（Sat PC）的合并量; B）选定研究的磷脂组成; c）放射性标记的前体掺入St PC和表面活性剂分泌动力学测量; d）通过气管内注射给予的放射性标记的表面活性剂组分（Sat PC、SP-A、SP-B、SP-C）的清除动力学。这些测量提供了有关表面活性剂流入和流出肺泡的信息。2. 通过测量a）最小和最大表面张力; B）表面活性剂的表面张力降低特性对血浆蛋白抑制的敏感性;和c）吸收率，在体外评价表面活性剂的表面活性。上述测量将使用Wilhelmy天平和/或捕获气泡表面张力计。3.将通过给予从表面活性剂缺乏的27天妊娠早产兔的小鼠肺泡泡中分离的表面活性剂来体内测量表面活性剂功能。将量化测试表面活性剂对早产兔肺的通气期间的动态顺应性和静态压力容积曲线的影响。

This core will assess surfactant metabolism a and surfactant function in the normal and transgenic mouse models used for the projects. All techniques to be used in this Core have been previously published in studies using transgenic mice. Four techniques to be used in this core have been previously published in studies using transgenic mice. Four types of measurements of surfactant metabolism and function will be provided to the projects: 1. Metabolic measurements of surfactant in vivo will include a) pool sizes of saturated phosphatidylcholine (Sat PC) in alveolar lavage and lung tissue; b) phospholipid composition for selected studies; c) radiolabeled precursor incorporation into St PC and measurements of the kinetics of surfactant secretion; and d) clearance kinetics for radiolabeled surfactant components (Sat PC, SP-A, SP-B, SP-C) given by intratracheal injection. These measurements provide information on the flux of surfactant into and out of the alveolus. 2. The surface activity of surfactant will be evaluated in vitro by measurements of a) minimum and maximum surface tension: b) sensitivity of surface tension lowering properties of surfactant to inhibition by plasma protein; and c) absorption rate. The Wilhelmy balance and/or captive bubble surfactometer will be used for above measurements. 3. Surfactant function will be measured in vivo by giving surfactant isolated from alveolar laves of mice to be surfactant deficient 27 d gestation premature rabbits. The effects of the test surfactants on dynamic compliance during ventilation and static pressure volume curves of the premature rabbit lung will be quantified.