NEUROGENESIS AND GLIOGENESIS IN THE DEVELOPING BRAIN

我们已经在人胎脑中鉴定出细胞 保持神经干或神经前体细胞形态的培养 细胞。这些细胞聚集在一起，被描述为神经球。在……里面 为了识别潜在的干细胞或祖细胞，我们有 制备抗人巢蛋白多肽的多克隆抗体 吉恩。巢蛋白是一种中间细丝，在 神经系统和其他组织中的祖细胞。 人胎脑内巢蛋白的免疫细胞化学检测 培养物与巢蛋白使用的mRNA鉴定相关 同胞培养中的RT-PCR检测。这些细胞也不与其他细胞发生反应 表型标记用于更成熟、分化的细胞 大脑。神经球中的一些细胞从 簇状排列，看起来会改变形态 取决于培养物的生长条件。细胞培养在 添加胎牛的标准最低必需培养液 血清表现为胞浆宽阔，胞核增大。这些细胞会产生反应 对胶质纤维酸性蛋白、GFAP抗体呈强阳性反应， 将这些细胞鉴定为星形胶质细胞。这些细胞也容易感染 感染人类多瘤病毒JCV，它感染人的细胞 由于细胞特异性基因，胶质细胞谱系不是神经元谱系 核因子-1/D转录因子的识别。使用这种病毒来 在分化过程中对细胞进行标记可能会为 询问细胞分化途径的实验 中枢神经系统。

We have identified cells in human fetal brain cultures that maintain the morphology of neural stem or progenitor cells. These cells cluster in aggregates described as neurospheres. In order to identify potential stem or progenitor cells, we have produced polyclonal antibodies to peptides from the human nestin gene. Nestin is an intermediate filament which is highly expressed in progenitor cells in the nervous system and other tissues. Immunocytochemical detection of nestin in human fetal brain cultures correlates with identification of mRNA for nestin using rt-PCR in sibling cultures. These cells also do not react with other phenotypic markers used for more mature, differentiated cells in the brain. Some of the cells in the neurospheres migrate from the clustered arrangement and appear to alter morphology depending upon the growth conditions of the cultures. Cells cultured on standard minimum essential medium supplemented with fetal bovine serum show broad cytoplasm with enlarged nuclei. These cells react strongly with antibody to glial fibrillary acidic protein, GFAP, identifying the cells as astrocytes. These cells also are susceptible to infection by the human polyomavirus, JCV, which infects cells in a glial lineage not a neuronal lineage because of cell specific gene recognition by NF-1/D transcription factor. The use of this virus to mark cells during differentiation may provide a new tool for experiments asking questions of pathways for cell differentiation in the CNS.