Development of an Embryo Cryopreservation Program in Laboratory Animals

实验动物胚胎冷冻保存计划的开发

• 批准号: 6112691
• 负责人: PATRICIA M SCHMIDT
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6112691
• 关键词: Rodentias; animal tissue; cell bank /registry; cryopreservation; embryo /fetus cell /tissue; genetic strain; genotype; laboratory rabbit; species difference; tissue resource /registry

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species, and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases. Genotype and species differences continue to impact on the efficiency of this is effort. (This is a continuation of Intramural Research Project Z01-RR-00077-13 VR.)

NIH动物遗传资源(NIHAGR) 保留了几个动物物种和许多无价的基因类型 在生物医学研究中被用作模型的物种。 为了保护这种种质，建立了一个胚胎库 无限期保存这些种群和品种的经济手段 啮齿动物和兔子。胚胎冷冻保存有其他作用 基因型管理的好处，例如防止丢失 来自疾病、生殖障碍的种质，自然的 灾难或基因污染。低温库将最小化 由于遗传漂移而产生的变化，通过提供一种资源 定期重新派生已定义的基因类型，以便重新标准化 基础繁育蜂群。作为公认的存储库 作为实验动物物种，NIHAGR通常提供繁殖 应要求在整个生物医学研究社区提供核。 与其运送活体动物，冷冻保存的胚胎代表着 一种更简单、更人性化和更容易运输请求的方法 世界各地的种质。胚胎的另一个重要影响 搬运、冷冻保存和转移程序是为了消除 一些鼠类疾病的垂直传播。基因型和 物种差异继续影响这一系统的效率 努力。(这是《校内研究计划》的延续 Z01-RR-00077-13 VR。)