DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 6290678
• 负责人: PATRICIA M SCHMIDT
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6290678
• 关键词: Rodentias; animal tissue; cell bank /registry; cryopreservation; embryo /fetus cell /tissue; genetic strain; genotype; laboratory rabbit; species difference; tissue resource /registry

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species, and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases. Genotype and species differences continue to impact on the efficiency of this is effort. - mouse embryos, cryopreservation, embryo banking, animal models

NIH动物遗传资源（NIHAGR）保存了几个动物物种，以及一个物种中许多宝贵的基因型，这些基因型被用作生物医学研究的模型。为了保护这一种质，建立了胚胎库，作为无限期保存这些啮齿动物和兔子种群和品系的经济手段。胚胎冷冻保存对基因型管理还有其他好处，如防止疾病、生殖失败、自然灾害或遗传污染造成的种质损失。冷冻库通过提供一种资源来最大限度地减少由于遗传漂变而引起的变化，从该资源中周期性地重新推导出定义的基因型，以重新标准化基础育种群体。作为公认的实验室动物物种库，NIHAGR根据整个生物医学研究界的要求定期提供育种细胞核。冷冻保存的胚胎代表了一种更简单，更人道，更容易在全球范围内运输所需种质的方法，而不是运输活体动物。胚胎处理、冷冻保存和移植程序的另一个重要影响是消除了一些鼠类疾病的垂直传播。基因型和物种差异继续影响这一努力的效率。- 小鼠胚胎，冷冻保存，胚胎库，动物模型