HRMAS OF RESIN BOUND OLIGOSACCHARIDES

人们已经认识到，低聚糖的形式是 糖蛋白和糖脂结合物，携带详细的结构 用于调解各种生物事件的信息 包括炎症、免疫反应和转移。而当 一些碳水化合物结合蛋白被发现是细胞表面的受体 细胞表面，它们的碳水化合物配体还没有确定。 鉴定这些碳水化合物配体将是至关重要的。 对理解碳水化合物参与细胞的兴趣 发信号。为了简化非常劳动密集型的 碳水化合物的溶液相合成，已经有了相当大的努力 被引导到发展战略和序列 与固相合成有关。最近的进展表明 在溶液中进行糖苷化的有用方法可以是 适用于聚合物支撑体。的一个主要限制 固体载体合成新方法的研究进展 复杂的低聚碳水化合物是表征 反应产物或中间产物，因为它们在 合成的过程中。直到最近，乳沟还是有必要的 这些产品是从树脂中提取出来的，以便使用 经典光谱方法(例如，溶液状态核磁共振和质量 光谱分析)，用于监测展开合成。乳沟 分析方法费时、昂贵、浪费。 多步合成的背景。我们最近介绍了使用 高分辨率魔角旋转(HR-MAS)核磁共振 聚合物结合低聚糖的表征。这项监控 方法使我们能够快速开发新的方法来 生物过程中涉及的复杂糖偶联物的组装。 最终，这些方法可能会导致开发自动化的 低聚糖合成器。

It has been recognized that oligosaccharides, in the form of glycoprotein and glycolipid conjugates, carry detailed structural information that serves to mediate a variety of biological events including inflammation, immunological response and metastasis. While some carbohydrate binding proteins were found to be receptors on the cell surface, their carbohydrate ligands have not yet been determined. Identification of these carbohydrate ligands would be of paramount interest in understanding the involvement of carbohydrates in cell signaling. In order to simplify the very labor-intensive solution-phase synthesis of carbohydrates, considerable efforts have been directed toward the development of strategies and sequences relevant to solid phase synthesis. Recent advances have demonstrated that useful methodologies for glycosidation in solution can be applicable on a polymer support. A major limitation toward the development of new methodologies for solid support synthesis of complex oligosacharides is the difficulty of characterizing the reaction products or intermediates as they are evolving during the course of the synthesis. Until recently, it was necessary to cleave these products from the resin in order to allow for the use of classical spectroscopic means (e.g. solution-state NMR and mass spectrometry) for monitoring the unfolding syntheses. The cleavage method of analysis is time consuming, expensive and wasteful in the context of multistep syntheses. We recently introduced the use of high-resolution magic angle spinning (HR-MAS) NMR for the characterization of polymer bound oligosaccharides. This monitoring method has allowed us to rapidly develop novel methodologies for the assembly of complex glycoconjugates involved in biological processes. Ultimately, these methods may lead to the development of an automated oligosaccharide synthesizer.