HRMAS OF RESIN BOUND OLIGOSACCHARIDES

已经认识到，低聚糖，以低聚糖的形式， 糖蛋白和糖脂结合物，携带详细的结构 用于调节各种生物事件的信息 包括炎症、免疫反应和转移。 而 一些碳水化合物结合蛋白被发现是在 细胞表面，其碳水化合物配体尚未确定。 这些碳水化合物配体的鉴定将是至关重要的 有兴趣了解碳水化合物参与细胞 发信号。 为了简化劳动密集型的 关于碳水化合物的液相合成， 一直致力于发展战略和序列， 与固相合成有关。 最近的进展表明， 在溶液中进行糖苷化的有用方法可以 可应用于聚合物载体上。 一个主要的限制， 固体载体合成的新方法学的发展 复杂的低聚糖是表征 反应产物或中间体，因为它们在反应过程中形成， 合成的过程。 直到最近， 这些产品从树脂，以允许使用 经典的光谱手段（例如溶液状态NMR和质谱） 光谱法）用于监测解折叠合成。 裂解 分析方法是耗时的，昂贵的和浪费的， 多步合成的背景。 我们最近介绍了使用 高分辨率魔角旋转（HR-MAS）NMR 聚合物结合寡糖的表征。 这一监测 方法使我们能够快速开发新的方法， 参与生物过程的复杂糖缀合物的组装。 最终，这些方法可能会导致自动化的开发 低聚糖合成器

It has been recognized that oligosaccharides, in the form of glycoprotein and glycolipid conjugates, carry detailed structural information that serves to mediate a variety of biological events including inflammation, immunological response and metastasis. While some carbohydrate binding proteins were found to be receptors on the cell surface, their carbohydrate ligands have not yet been determined. Identification of these carbohydrate ligands would be of paramount interest in understanding the involvement of carbohydrates in cell signaling. In order to simplify the very labor-intensive solution-phase synthesis of carbohydrates, considerable efforts have been directed toward the development of strategies and sequences relevant to solid phase synthesis. Recent advances have demonstrated that useful methodologies for glycosidation in solution can be applicable on a polymer support. A major limitation toward the development of new methodologies for solid support synthesis of complex oligosacharides is the difficulty of characterizing the reaction products or intermediates as they are evolving during the course of the synthesis. Until recently, it was necessary to cleave these products from the resin in order to allow for the use of classical spectroscopic means (e.g. solution-state NMR and mass spectrometry) for monitoring the unfolding syntheses. The cleavage method of analysis is time consuming, expensive and wasteful in the context of multistep syntheses. We recently introduced the use of high-resolution magic angle spinning (HR-MAS) NMR for the characterization of polymer bound oligosaccharides. This monitoring method has allowed us to rapidly develop novel methodologies for the assembly of complex glycoconjugates involved in biological processes. Ultimately, these methods may lead to the development of an automated oligosaccharide synthesizer.