DIRECT EXAMINATION OF SEPARATION PROCESSES BY LASER INDUCED FLUORESCENCE

通过激光诱导荧光直接检查分离过程

• 批准号: 6258838
• 负责人: VICTORIA L MCGUFFIN
• 金额: $ 0.01万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258838
• 关键词: biological products; biomedical resource; enzymes; neoplasm /cancer; proteins; technology /technique development

Polycyclic aromatic compounds (PACs) are an important group of pollutants which are found at trace levels throughout the environment. They consist of two or more fused aromatic rings, with a large number of isomeric forms. In addition, subtle changes in structure can result in dramatic changes in their observed chemical and toxicological properties. Together they form the largest known class of chemical carcinogens and mutagens, with sixteen PACs consisting of only cabon and hydrogen (polycyclic aromatic hydrocarbons or PAHs) identified by the EPA. One method which can discriminate between various structural classes of PACs is the use of selective quenching agents in fluorescence spectroscopy. In a quenching approach, an excited-state fluorophore is deliberately deactivated by a collision with a quencher that selectively promotes nonradiative relaxation. This method can provide valuable photophysical and photochemical information about the individual fluorophore that can be used for classification or identification. In addition, when combined with separation methods it can be used to simplify the qualitative and quantitative analysis of complex samples by selective discrimination against interfering components. This project is concerned with evaluating the effectiveness and selectivity of novel quenchers of PACs. Given the nature of the experiments, the purity of both fluorophores and quenchers is important. Mass spectrometry can be used to determine the presence and identity of impurities, which in turn helps determine the best method for their removal. MS analysis of the purified reagents is carried out to confirm the absence of impurities before experimental work can be begun.

多环芳香族化合物（PAC）是一类重要的芳香族化合物， 在整个环境中发现的痕量污染物。 它们由两个或多个稠合的芳环组成，其中大量的 异构形式。 此外，结构上的细微变化可以 导致其观察到的化学物质发生巨大变化， 毒理学特性 它们共同构成了已知最大的类 化学致癌物和诱变剂，有16个PAC， 只有碳和氢（多环芳烃或PAH） 由EPA认证。 有一种方法可以区分 PAC的各种结构类别是使用选择性猝灭 荧光光谱学中的试剂。 在淬火方法中， 激发态荧光团被有意地通过碰撞而失活 与选择性促进非辐射弛豫的猝灭剂一起。 该方法可提供有价值的光物理和光化学方法 关于可用于以下的单个荧光团的信息： 分类或识别。 此外，当与 分离方法，它可以用来简化定性和 复杂样品的选择性鉴别定量分析 防止干扰组件。 本项目涉及 评价新型猝灭剂的有效性和选择性， PAC。 考虑到实验的性质， 荧光团和猝灭剂是重要的。 质谱法可以是 用于测定杂质的存在和鉴别， 这有助于确定最佳的去除方法。 MS分析 进行纯化试剂的检测，以确认不存在 在实验工作开始之前，必须先去除杂质。