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ATP COUPLED ARSENICAL PUMP OF E COLI

ATP COUPLED ARSENICAL PUMP OF E COLI
大肠杆菌的 ATP 偶联砷泵
批准号:
6258843
负责人:
ROBERT A MITCHELL
金额:
$0.01万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-06-01 至 1999-11-30

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中文摘要
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英文摘要
We are studying the hydrolysis of ATP catalyzed by ArsA ATPase to see if it catalyses an phosphate-water oxygen exchange analogous to that of mitochonrial F1 ATPase. Studies on the latter have provided important information on the relation between ATP hydrolysis and energy coupling and on site-site interactions (P. Boyer's binding change mechanism). No exchange was detected at high concentrations of ATP and we are now using low levels of ATP to try to examine ATP produced by single site turnover. This is now possible because of the availability of larger amounts of purified ArsA protein.
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