EFFECT OF HYPERGLYCEMIA ON ERYTHROCYTE TRANSMEMBRANE ASYMMETRY

糖尿病发病率和死亡率主要是由于 涉及微血管闭塞事件的并发症。 此外，委员会认为， 糖尿病红细胞膜磷脂不对称性 被扰乱;氨基磷脂被发现在增加的数量， 质膜外层单层。 我们复制了这种效果 通过在体外用高血糖处理非糖尿病红细胞 葡萄糖浓度。 葡萄糖诱导的不对称性丧失是一种 这是氧化剂介导的被动脂质触发器增加的结果。 目前的研究旨在确定化学和物理 调节这种膜扰动的机制。 新战略 心血管并发症的防治 糖尿病患者可能导致这项工作。 脂质触发器的测量具有 需要合成具有短酰基链的磷脂。 的 通过FAB-MS对这些脂质进行了分析， 测量.

Diabetic morbidity and mortality is largely a result of complications involving microvascular occlusive events. Furthermore, transmembrane phospholipid asymmetry in human diabetic red blood cells is perturbed; aminophospholipids are found in increased quantity in the plasma membrane outer monolayer. We have duplicated this effect in vitro by treating non-diabetic red cells with hyperglycemic concentrations of glucose. The glucose-induced loss of asymmetry is a result of an oxidant-mediated increase in passive lipid flip-flop. Current studies are aimed at determining the chemical and physical mechanisms mediating this membrane perturbation. New strategies for the prevention and treatment of cardiovascular complications in diabetics may result this work. Measurements of lipid flip-flop have required the synthesis of phospholipids with short acyl chains. The analysis of these lipids has been performed by FAB-MS and exact mass measurement.