Digital design and fabrication of advanced biopurification materials

先进生物净化材料的数字化设计和制造

• 批准号: MR/W004399/1
• 负责人: Daniel Bracewell
• 金额: $ 32.67万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FW004399%2F1
• 关键词: Digital; design; fabrication; advanced; biopurification

The global need for complex biological medicines such as cancer treatments and vaccines continues to grow. However the capability to manufacture high quality products at scale remains challenging with existing technology. Improvements to production capabilities and demand for products based on viral vectors and liposomes have resulted in exacerbated purification issues downstream, leading to performance and lifespan compromises of biomanufacturing unit operations such as filtration and chromatography. Solutions are required in order purify ever increasingly concentrated process streams in an efficient and cost-effective manner to meet growing demand for bio-therapeutics.The research proposed here utilises cutting-edge 3D imaging, analysis and digital design approaches in order to produce novel bioseparation materials that have clear advantages over existing purification technology. Applying methodology established at UCL structures can be designed, fabricated through 3D printing, analysed and improved through a design cycle. Pall Biotech, a leading international bioprocess vendor and applications expert, will provide secondment opportunities that will enable access to industrial experts and industrially relevant materials for this research in order to perform commercially applicable case studies.

全球对癌症治疗和疫苗等复杂生物药物的需求持续增长。然而，利用现有技术，大规模生产高质量产品的能力仍然具有挑战性。对基于病毒载体和脂质体的产品的生产能力和需求的改进导致下游纯化问题加剧，导致生物制造单元操作（如过滤和色谱）的性能和寿命受损。为了以高效和经济的方式净化日益浓缩的工艺流，以满足日益增长的生物治疗需求，需要解决方案。这里提出的研究利用尖端的3D成像，分析和数字设计方法，以生产新型生物分离材料，这些材料比现有的净化技术具有明显的优势。应用UCL建立的方法，结构可以通过3D打印设计，制造，分析和改进。Pall Biotech是一家领先的国际生物工艺供应商和应用专家，将提供借调机会，以便获得工业专家和与本研究相关的工业材料，以便进行商业上适用的案例研究。

项目成果

High-resolution imaging of depth filter structures using X-ray computed tomography

• DOI: 10.1007/s10853-021-06238-w
• 发表时间: 2021-06-18
• 期刊: JOURNAL OF MATERIALS SCIENCE
• 影响因子: 4.5
• 作者: Johnson, T. F.;Iacoviello, F.;Bracewell, D. G.
• 通讯作者: Bracewell, D. G.

Analysis of fouling and breakthrough of process related impurities during depth filtration using confocal microscopy.

• DOI: 10.1002/btpr.3233
• 发表时间: 2022-03
• 期刊: BIOTECHNOLOGY PROGRESS
• 影响因子: 2.9
• 作者: Parau, Maria;Johnson, Thomas F.;Pullen, James;Bracewell, Daniel G.
• 通讯作者: Bracewell, Daniel G.

Evaluating 3D-printed bioseparation structures using multi-length scale tomography.

• DOI: 10.1007/s00216-023-04866-6
• 发表时间: 2023-10
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Johnson, Thomas F.;Conti, Mariachiara;Iacoviello, Francesco;Shearing, Paul R.;Pullen, James;Dimartino, Simone;Bracewell, Daniel G.
• 通讯作者: Bracewell, Daniel G.

Evaluating 3D printed bioseparation structures using multi-length scale tomography

使用多长度尺度断层扫描评估 3D 打印的生物分离结构

• DOI: 10.22541/au.167587574.46873452/v1
• 发表时间: 2023
• 作者: Johnson T

Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy.

• DOI: 10.3390/membranes11110905
• 发表时间: 2021-11-22
• 期刊: Membranes
• 影响因子: 4.2
• 作者: Johnson TF;Jones K;Iacoviello F;Turner S;Jackson NB;Zourna K;Welsh JH;Shearing PR;Hoare M;Bracewell DG
• 通讯作者: Bracewell DG