UREMIA, ACIDOSIS & DIALYSIS ON PROTEIN METABOLISM: LONGITUDINAL LEUCINE KINETICS
UREMIA, ACIDOSIS & DIALYSIS ON PROTEIN METABOLISM: LONGITUDINAL LEUCINE KINETICS
批准号:
6118556
负责人:
VICTORIA S LIM
金额:
$0.16万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-08-01 至 1999-07-31
中文摘要
尿毒症和透析被认为是分解代谢过程导致
英文摘要
Uremia and dialysis are viewed as catabolic processes resulting in
malnutrition in chronic renal failure (CRF) patients. To sort out the
effects of uremia, acidosis and dialysis on protein metabolism, we
measured leucine flux in CRF patients before and after initiation of
maintenance dialysis. Whole body leucine flux was measured by
primed-constant infusion of L[1-13C]leucine in 9 CRF patients
longitudinally; twice before and once after initiation of maintenance
dialysis [D]. Before dialysis, one leucine flux was measured when the
patients were acidotic [A], and the other, when acidosis was corrected
with NaHCO3[NA]. Five normal subjects underwent one single leucine
flux measurement to serve as control [N]. Both patients and normal
subjects consumed a constant diet for 6 days and leucine flux was
measured on the 7th day 12 hr. post-absorption. Diet for the CRF
patients was identical during the three periods. Plasma L[1-13C]
leucine and L[1-13C]KIC were measured by gas c hromatography/mass
spectrometry and expired 13CO2 by isotope ratio spectrometry. Leucine
kinetics were calculated using standard equations. Plasma CO2 were
19,26 and 31 mmol/L in A, NA and D periods, respectively. All kinetic
results ((mol/kg/hr) are presented as means (SD in the order of A, NA,
D and N, and CRF values that are statistically different from N are
identified [*]. The amount of leucine release from endogenous protein
breakdown [Ra or Q] were 101(12*, 95(9*, 113(22 and 117(6. Leucine
oxidation [C], quantity of leucine irreversibly oxidized to CO2, were
16.5(5.4, 9.7(3.7*, 12.3(3.0* and 23.2(3.1. Leucine protein
incorporation [S] were 85(10, 85(8, 101(19 and 94(6. The S of 101 in
CRF patients at period D was statistically higher than those during A
and NA periods. These data indicate that when acidosis was corrected,
CRF patients adapted to lower protein intake by reducing amino acid
oxidation and protein degradation, and maintained protein synthesis at
normal level . Metabolic acidosis impaired the down regulation of
amino acid oxidation. Maintenance dialysis treatment longitudinally
restored protein flux to normal and increased protein synthesis. The
general notion that uremia and dialysis are protein catabolic are not
supported by this work.
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会议论文
REQUEST TO TAKE BLOOD SAMPLES FROM 20 HEALTHY CONTROL SUBJECTS
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批准号:7604886
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项目类别:
-
资助金额:$0.01万
-
财政年份:2007
-
负责人:VICTORIA S LIM
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依托单位:
LEUCINE TRNOVR RATS W/ NEPHROTIC SYNDROME SUGGEST BODY PROTEIN CONSERVATION
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批准号:6665861
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项目类别:
-
资助金额:$15.75万
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财政年份:2002
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负责人:VICTORIA S LIM
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依托单位:
PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS
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批准号:6665862
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项目类别:
-
资助金额:$15.75万
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财政年份:2002
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负责人:VICTORIA S LIM
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依托单位:
PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS
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批准号:6486742
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项目类别:
-
资助金额:$15.75万
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财政年份:2001
-
负责人:VICTORIA S LIM
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依托单位:
LEUCINE TRNOVR RATS W/ NEPHROTIC SYNDROME SUGGEST BODY PROTEIN CONSERVATION
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批准号:6486741
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项目类别:
-
资助金额:$15.75万
-
财政年份:2001
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负责人:VICTORIA S LIM
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依托单位:
PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS
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批准号:6336812
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项目类别:
-
资助金额:$0.6万
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财政年份:2000
-
负责人:VICTORIA S LIM
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依托单位:
LEUCINE TRNOVR RATS W/ NEPHROTIC SYNDROME SUGGEST BODY PROTEIN CONSERVATION
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批准号:6336811
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项目类别:
-
资助金额:$0.6万
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财政年份:2000
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负责人:VICTORIA S LIM
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依托单位:
EFFECT OF UREMIA AND ACIDOSIS ON INSULIN INDUCED PROTEIN ANABOLISM
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批准号:6304819
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项目类别:
-
资助金额:$2.2万
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财政年份:1999
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负责人:VICTORIA S LIM
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依托单位:
EFFECT OF UREMIA AND ACIDOSIS ON INSULIN INDUCED PROTEIN ANABOLISM
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批准号:6114725
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项目类别:
-
资助金额:$2.2万
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财政年份:1998
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负责人:VICTORIA S LIM
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依托单位:
LEUCINE TURNOVER IN PATS W/ NEPHROTIC SYN: POSSIBLE BODY PROTEIN CONSERVATION
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批准号:6118555
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项目类别:
-
资助金额:$0.32万
-
财政年份:1998
-
负责人:VICTORIA S LIM
-
依托单位:
PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS
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批准号:6249748
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项目类别:
-
资助金额:$0.42万
-
财政年份:1997
-
负责人:VICTORIA S LIM
-
依托单位:
LEUCINE TURNOVER IN PATS W/ NEPHROTIC SYNDROME: BODY PROTEIN CONSERVATION
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批准号:6249747
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项目类别:
-
资助金额:$0.42万
-
财政年份:1997
-
负责人:VICTORIA S LIM
-
依托单位:
EFFECT OF UREMIA AND ACIDOSIS ON INSULIN INDUCED PROTEIN ANABOLISM
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批准号:6275960
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项目类别:
-
资助金额:$1.92万
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财政年份:1997
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负责人:VICTORIA S LIM
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依托单位:
EFFECT OF UREMIA AND ACIDOSIS ON INSULIN INDUCED PROTEIN ANABOLISM
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批准号:6245838
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项目类别:
-
资助金额:$1.92万
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财政年份:1997
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负责人:VICTORIA S LIM
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依托单位:
EFFECT OF UREMIA ON PROTEIN METABOLISM
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批准号:5218538
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:VICTORIA S LIM
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依托单位:--
PROTEIN TURNOVER IN PATIENTS W/ CHRONIC RENAL FAILURE: LEUCINE KINETIC STUDY
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批准号:5221821
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:VICTORIA S LIM
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依托单位:--
PROTEIN METABOLISM IN PATIENTS W/ CHRONIC RENAL FAILURE: LEUCINE KINETIC STUDY
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批准号:5221820
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项目类别:
-
资助金额:$0.0万
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财政年份:--
-
负责人:VICTORIA S LIM
-
依托单位:--
EFFECT OF UREMIA AND ACIDOSIS ON INSULIN INDUCED PROTEIN ANABOLISM
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批准号:5218618
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:VICTORIA S LIM
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依托单位:--
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