PORPHYRIN NUCLEIC ACID INTERACTIONS

我们制备了含有共价键的烷基卟啉的杂化物， 通过氧丙基接头嵌入寡脱氧核苷酸中， 骨架的磷酸基团。 这些使我们能够研究 结构类似的卟啉与普通卟啉之间的相互作用 辅因子;在自然界和DNA中发现。 （该领域的前期工作 几乎都是用异生物质 meso-四芳基卟啉）。 在不同的温度下， 记录序列5 ′-CGCGCCTTC-P-CATTGCGG-3 ′的温度， 其中-P-表示与互补DNA复合的卟啉 在面对卟啉的位置带有胸苷残基的链。 该双链体已被选为在许多DNA中最稳定的。 不同的情结。 在FBML的500 MHz上获得的光谱 光谱仪使我们能够得到定性结构 关于卟啉-DNA相互作用的信息。 连同其他 光谱和足迹数据，温度依赖的NMR 数据使我们能够提出大环的疝嵌 作为卟啉-DNA最有可能的结构结果 交互.

We have prepared hybrids containing an alkylporphyrin covalently embedded in oligodeoxynucleotides via oxypropyl linkers to the phosphate groups of the backbone. These have allowed us to study interactions between a porphyrin similar in structure to common cofactors; found in nature and DNA. (Previous work in this field has almost exclusively been performed with xenobiotic meso-tetraarylporphyrins). A series of NMR spectra at different temperatures were recorded of the sequence 5'-CGCGCCTTC-P-CATTGCGG-3', where -P- denotes the porphyrin, complexed with a complementary DNA strand bearing a thymidine residue at the site facing the porphyrin. This duplex had been selected as the most stable among a number of different complexes. The spectra acquired on the FBML's 500 MHz spectrometer have allowed us to derive qualitative structural information about the porphyrin-DNA interactions. Together with other spectroscopic and footprinting data, the temperature-dependent NMR data have allowed us to propose herniintercalation of the macrocycle as the most likely structural consequence of the porphyrin-DNA interactions.