ANALYSIS OF PROTEIN-PROTEIN COMPLEXES

蛋白质-蛋白质复合物的分析

• 批准号: 6279503
• 负责人: UROOJ A MIRZA
• 金额: $ 0.08万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279503
• 关键词: biomedical equipment development; biomedical resource; spectrometry; technology /technique

Conditions have been developed for directly detecting protein-protein complexes by electrospray ionization mass spectrometry. To date, we have successfully detected strongly associated complexes, such as the p42/p62 heterodimer. p42/p62 is a TBP associated factor (TAF) that shares sequence homology with histones H3/H4. We are currently trying to determine the relationship between our ability to measure non-covalent complexes in the gas phase and the strength of the associations between the same partners in solution. Partial results of this study were reported in Nature 38 (1996) 316322.

已经开发了直接检测 蛋白质-蛋白质复合物电喷雾质谱法 光谱法 到目前为止，我们已经成功地检测到强烈的 相关复合物，如p42/p62异二聚体。 p42/p62是一种 TBP相关因子（TAF）与 组蛋白H3/H4。 我们目前正试图确定 我们在气相中测量非共价复合物的能力 以及相同的合作伙伴之间的联系的强度， 溶液 这项研究的部分结果发表在Nature 38上 （1996）316322。