The cellular immune response to B.1.1.7 variant COVID-19 deciphered by single cell multi-omics

单细胞多组学破译对 B.1.1.7 变体 COVID-19 的细胞免疫反应

• 批准号: MR/W014556/1
• 负责人: Berthold Gottgens
• 金额: $ 4.75万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FW014556%2F1
• 关键词: cellular; immune; response; B.1.1.7; variant

Regardless of rapid vaccination programs, the coronavirus SARS-CoV2 is likely to remain a concern for the foreseeable future, because viral pathogens evolve, and acquire mutations that may alter severity of the disease or render current vaccines ineffective. During the 1st wave of coronavirus, we have analysed over 800,000 blood and immune cells from more than 100 patients treated in Cambridge, Newcastle and London. This work provides the largest reference dataset available anywhere to interrogate how blood and immune cells are affected by the infection, and may contribute to fighting it. Given the recent emergence of a more aggressive strain (the so-called UK or Kent variant), there is an urgent need to investigate whether this new variant causes the same changes in blood and immune cells as the original strain. Such information is vital to know whether current therapies need to be amended, and whether there are opportunities to develop new treatments. We propose to answer these important questions by studying 30 patients of the current wave, where the majority are infected with the new variant. Because of time sensitivity, our results will be made available to the wider scientific community as soon as is practicable.

无论快速疫苗接种计划如何，在可预见的未来，冠状病毒SARS-CoV 2可能仍然是一个令人担忧的问题，因为病毒病原体会进化，并获得可能改变疾病严重程度或使现有疫苗无效的突变。在第一波冠状病毒期间，我们分析了来自剑桥、纽卡斯尔和伦敦100多名患者的80多万个血液和免疫细胞。这项工作提供了最大的参考数据集，可以询问血液和免疫细胞如何受到感染的影响，并可能有助于对抗它。鉴于最近出现了一种更具侵略性的菌株（所谓的UK或肯特变种），迫切需要调查这种新变种是否会导致血液和免疫细胞发生与原始菌株相同的变化。这些信息对于了解目前的治疗方法是否需要修改以及是否有机会开发新的治疗方法至关重要。我们建议通过研究30名当前浪潮的患者来回答这些重要问题，其中大多数人感染了新的变体。由于时间敏感性，我们的结果将尽快提供给更广泛的科学界。

项目成果

Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans.

• DOI: 10.1182/blood.2021013450
• 发表时间: 2022-06-09
• 期刊: Blood
• 影响因子: 20.3
• 作者: Mende N;Bastos HP;Santoro A;Mahbubani KT;Ciaurro V;Calderbank EF;Quiroga Londoño M;Sham K;Mantica G;Morishima T;Mitchell E;Lidonnici MR;Meier-Abt F;Hayler D;Jardine L;Curd A;Haniffa M;Ferrari G;Takizawa H;Wilson NK;Göttgens B;Saeb-Parsy K;Frontini M;Laurenti E
• 通讯作者: Laurenti E

Blood and immune development in human fetal bone marrow and Down syndrome.

人类胎儿骨髓和唐氏综合症的血液和免疫发育。

• DOI: 10.17863/cam.76568
• 发表时间: 2021
• 作者: Jardine L

The impact of hypoxia on B cells in COVID-19.

• DOI: 10.1016/j.ebiom.2022.103878
• 发表时间: 2022-03
• 期刊: EBioMedicine
• 影响因子: 11.1
• 作者: Kotagiri P;Mescia F;Hanson AL;Turner L;Bergamaschi L;Peñalver A;Richoz N;Moore SD;Ortmann BM;Dunmore BJ;Morgan MD;Tuong ZK;Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration;Göttgens B;Toshner M;Hess C;Maxwell PH;Clatworthy MR;Nathan JA;Bradley JR;Lyons PA;Burrows N;Smith KGC
• 通讯作者: Smith KGC

Single-Cell Protein Analysis - Methods and Protocols

单细胞蛋白质分析 - 方法和实验方案

• DOI: 10.1007/978-1-0716-1771-7_13
• 发表时间: 2022
• 作者: Mende N

Local and systemic responses to SARS-CoV-2 infection in children and adults

儿童和成人对 SARS-CoV-2 感染的局部和全身反应

• DOI: 10.17863/cam.89258
• 发表时间: 2022
• 作者: Gottgens B