CRYSTALLOGRAPHIC ANALYSIS OF TERPENE CYCLASES

萜烯环化酶的晶体分析

• 批准号: 6119405
• 负责人: COURTNEY STARKS
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119405
• 关键词: Fungi; plants; proteins

Terpene cyclases catalyze the conversion of acyclic hydrocarbon diphosphates to a wide variety of cyclic skeletons, via readily rearranging carbocation intermediates. The enormous array of possible terpenoid structures provides a natural library of compounds to screen for pharmaceutical and agricultural applications, and natural and engineered terpene cyclases could provide readily available in vitro sources of these compounds. In order to better understand the machinery of terpene cyclases and how to re-engineer them, we have determined the structure of 5-epi-aristolochene synthase (TEAS), a terpene cyclase from tobacco. Based on this structure, we have designed several mutant enzymes, which have new substrate and product specificities. We are now further examining the determinants of specificity in TEAS and working to determine crystal structures of other terpene cyclases.

萜烯环酶催化非环烃的转化 二磷酸盐到各种各样的环状骨架，通过很容易 重新安排碳阳离子中间体。可能的巨大数组 萜类结构为筛选提供了一个天然的化合物文库 用于制药和农业应用，以及天然和 工程的萜烯环化酶可以在体外提供容易获得的 这些化合物的来源。为了更好地理解 萜类环化酶的机制以及如何重新设计它们，我们有 测定了5-表马兜铃烯合成酶(TEAS)的结构。 烟草中的萜类环化酶。基于这个结构，我们有 设计了几种突变酶，它们具有新的底物和产物 具体细节。我们现在正在进一步研究决定因素 茶的专一性和测定其晶体结构的工作 其他萜类环化酶。