STRUCTURES OF DNA REPAIR ENZYME DNA COMPLEXES

DNA 修复酶 DNA 复合物的结构

• 批准号: 6119424
• 负责人: CLIFFORD D MOL
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119424
• 关键词: biomaterials; genome; human tissue; neoplasm /cancer; nucleic acids

DNA repair enzymes play a key role in maintaining the genonmic integrity of all living cells. They are responsible for detecting and excising the potentially mutagenic lesions that arise in DNA either spontaneously or via oxidative damage. Detailed, high-resolution X-ray crystal structures of these enzymes, and their DNA complexes, will provide a solid basis towards understanding the mechanistic and biologic ramifications of essential repair processes. The DNA repair enzyme that is the subject of this proposal is human uracil-DNA glycosylase (UDG), and we have collected several high-resolution (>2.0 E) data sets of both wild-type and mutant human UDG bound to uracil-containing duplex DNA at SSRL beamline 7-1. The determination of these structures revealed in atomic detail the basis for UDG specificity and suggested models for initial DNA damage detection.

DNA修复酶在维持基因组完整性方面起着关键作用