STRUCTURES OF DNA REPAIR ENZYMES & ENZYME DNA COMPLEXES

DNA修复酶在维持基因组中起着关键作用 所有活细胞的完整性。他们负责检测和 切除DNA中可能出现的突变损伤 自发地或通过氧化损伤。详细的x射线晶体 这些酶及其DNA复合体的结构，将提供 为理解机制和生物学奠定坚实的基础 基本维修流程的影响，并将协助 具有潜在药理意义的化合物的设计 广泛的化学和放射治疗应用。结晶试验 与核酸内切酶IV和不同的寡核苷酸复合体 所有四种酶都在进行中。

DNA repair enzymes play a key role in maintaining the genomic integrity of all living cells. They are responsible for detecting and excising the potentially mutagenic lesions that arise in DNA either spontaneously or via oxidative damage. Detailed x-ray crystal structures of these enzymes and their DNA complexes, will provide a solid basis toward understanding the mechanisms and biologic ramifications of essential repair processes and will assist in the design of pharmacologically significant compounds with potentially vast chemo- and radiotherapeutic applications. Crystallization trials with endonuclease IV and with different oligonucleotide complexes of all four enzymes are underway.