STRUCTURES OF DNA REPAIR ENZYMES & ENZYME DNA COMPLEXES

DNA 修复酶的结构

• 批准号: 6437475
• 负责人: CLIFFORD D MOL
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437475
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

DNA repair enzymes play a key role in maintaining the genomic integrity of all living cells. They are responsible for detecting and excising the potentially mutagenic lesions that arise in DNA either spontaneously or via oxidative damage. Detailed x-ray crystal structures of these enzymes and their DNA complexes, will provide a solid basis toward understanding the mechanisms and biologic ramifications of essential repair processes and will assist in the design of pharmacologically significant compounds with potentially vast chemo- and radiotherapeutic applications. Crystallization trials with endonuclease IV and with different oligonucleotide complexes of all four enzymes are underway.

DNA修复酶在维持基因组中起着关键作用。 所有活细胞的完整性 他们负责检测和 切除DNA中出现的潜在诱变损伤， 自发或通过氧化损伤。 详细的X射线晶体 这些酶及其DNA复合物的结构，将提供一个 为理解机制和生物学奠定坚实的基础 必要的维修过程的后果，并将协助 设计具有潜在重要性的化合物 巨大的化学和放射性应用。 结晶试验 与核酸内切酶IV和与不同的寡核苷酸复合物 所有四种酶都在进行中。