STAFF PRIORITY TIME EFFECTS OF MICROGRAVITY ON CRYSTAL GROWTH

我们收集了在微重力下生长的胰岛素晶体的数据。 航天飞机上的条件和地球上的条件在其他方面相同 评估微重力对晶体影响的条件(如果有的话) 成长。我们还感兴趣的是关于 蛋白质晶体的性质。我们在非聚焦模式下使用了光束线1-5 为了提供高度平行且单色的光束以便 收集受乐器影响影响最小的反射剖面。 通过使用Quantum4CCD探测器，我们能够收集2度 来自两个系列中的每一个的非常窄(0.001度)的图像条带 微重力和研磨的胰岛素晶体。水晶已经安装好了 在毛细血管中，以避免冷冻伪影。首字母 对数据的分析表明，各个反射剖面是 非常窄(0.005度)的个别山峰的加剧。这些数据是 现在正在进行更仔细的分析。我们还调查了 通过在不同帧重复采集相同数据进行精细切片 宽度来研究精细切片的好处。我们还做了一些 深冷条件下反射剖面的初步研究 水晶。

We collected data from insulin crystals grown under microgravity conditions on the space shuttle and on earth under otherwise identical conditions to assess the effects, if any, of microgravity on crystal growth. We are also interested in more general questions about the nature of protein crystals. We used beamline 1-5 in unfocussed mode to provide a highly parallel and monochromatic beam in order to collect reflection profiles minimally altered by instrumental affects. By using the Quantum4 CCD detector we were able to collect a 2 degree swath of very narrow (0.001 deg) images from each of a series of both microgravity and ground insulin crystals. The crystals were mounted in capillaries in order to avoid cryocooling artifacts. Initial analysis of the data shows the individual reflection profiles to be aggragates of very narrow (0.005 deg) individual peaks. The data are now being analyzed more closely. We also investigated the effect of fine slicing by collecting the same data repeatedly at different frame widths to investigate the benefits of fine slicing. We also did some preliminary investigations of reflection profiles from cryocooled crystals.