STAFF PRIORITY TIME EFFECTS OF MICROGRAVITY ON CRYSTAL GROWTH

工作人员优先考虑微重力对晶体生长的时间影响

• 批准号: 6119371
• 负责人: HENRY BELLAMY
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119371
• 关键词: Fungi; biomaterials; genome; plants; proteins

We collected data from insulin crystals grown under microgravity conditions on the space shuttle and on earth under otherwise identical conditions to assess the effects, if any, of microgravity on crystal growth. We are also interested in more general questions about the nature of protein crystals. We used beamline 1-5 in unfocussed mode to provide a highly parallel and monochromatic beam in order to collect reflection profiles minimally altered by instrumental affects. By using the Quantum4 CCD detector we were able to collect a 2 degree swath of very narrow (0.001 deg) images from each of a series of both microgravity and ground insulin crystals. The crystals were mounted in capillaries in order to avoid cryocooling artifacts. Initial analysis of the data shows the individual reflection profiles to be aggragates of very narrow (0.005 deg) individual peaks. The data are now being analyzed more closely. We also investigated the effect of fine slicing by collecting the same data repeatedly at different frame widths to investigate the benefits of fine slicing. We also did some preliminary investigations of reflection profiles from cryocooled crystals.

我们从微重力下生长的胰岛素晶体收集了数据 航天飞机和地球上的条件在其他相同的情况下 评估微重力对晶体的影响（如果有的话）的条件 生长。 我们也对有关有关的更一般性问题感兴趣 蛋白质晶体的性质。 我们在未关注的模式下使用了梁线1-5 提供高度平行和单色的光束，以便 收集反射曲线通过仪器影响最小化。 通过使用Quantum4 CCD检测器，我们能够收集2度 一系列一系列的非常狭窄的图像（0.001度）图像 微重力和地面胰岛素晶体。 晶体安装 在毛细管中，以避免冷冻文物。 最初的 数据的分析表明单个反射曲线为 加剧非常狭窄（0.005度）的单个峰。 数据是 现在正在更仔细地进行分析。 我们还调查了 通过在不同框架上重复收集相同的数据来进行细切片 宽度以调查细切片的好处。 我们也做了一些 初步研究冷冻的反射曲线 晶体。