STAFF PRIORITY TIME CRYSTAL STRUCTURE OF ARSENITE OXIDASE

亚砷酸盐氧化酶的工作人员优先时间晶体结构

• 批准号: 6119368
• 负责人: PETER KUHN
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119368
• 关键词: biomaterials; proteins

Arsenite oxidase from Alcaligenes faecalis is an 85 kDa protein induced by the presence of AsO2- (arsenite). It functions to oxidise AsIIIO2- to AsVO43- (arsenate), which, although it is a structural analogue of phosphate and inhibits phosphorylation by producing unstable arsenylated derivatives, is less toxic than AsO2-, which binds to essential sulfhydryl groups of proteins and dithiols such as glutaredoxin, Arsenite oxidase was isolated from A. faecalis by Anderson et al. at Ohio State University. Spectroscopic studies showed that the protein contains one molybdenum, five or six irons, and inorganic sulfide, where the molybdenum appears to be in the form of a molybdopterin and the irons one [4Fe-4S] HiPIP or [3Fe-4S] cluster and one Rieske-type [2Fe-2S] cluster. Together with the electron acceptors azurin and cytochrome c (E0;320 mV), arsenite oxidase provides a relatively high potential electron transfer system for detoxification of arsenite (E0;60 mV) to arsenate. We have successfully grown seven crystal forms (P1, P2, C2, I222, P4, P41212 and R32) from preparations of arsenite oxidase. Depending on the form, the limit of diffraction is between 3 E and approximately 1.2 E. We have collected data sets from the P2, I222, P4 and P41212 forms but have concentrated on the P1 form, which, although it is almost invariably twinned, is the most reproducible. We have collected data to 2.10 E from native P1 arsenite oxidase. Analysis of the self-rotation function and the plausible solvent content of the cell (a=91 E, b=110 E, c=118 E, a=98 , _=90 , and ?=96 ) indicated that the cell contains four unique molecules.

来自粪产碱菌的亚砷酸盐氧化酶是85 kDa的蛋白质 由AsO 2-（亚砷酸盐）的存在引起。 它的功能是氧化 AsIIIO 2-到AsVO 43-（砷酸盐），虽然它是结构性的， 磷酸盐的类似物，并通过产生 不稳定的砷化衍生物，毒性低于AsO 2-， 与蛋白质和二硫醇的必需巯基结合， 从A.粪便通过 安德森等人在俄亥俄州州立大学。 光谱研究 表明蛋白质含有一个钼，五个或六个铁， 和无机硫化物，其中钼似乎是在形式 钼蝶呤和铁[4 Fe-4S] HiPIP或[3 Fe-4S] [2Fe-2S]簇合物。 连同 电子受体天青蛋白和细胞色素c（E0;320 mV），亚砷酸盐 氧化酶提供相对高电位的电子转移系统 用于将亚砷酸盐（E0;60 mV）解毒为砷酸盐。 我们有 成功地生长了七种晶型（P1、P2、C2、I222、P4、P41212 和R32）。 取决于 衍射极限在3E到大约1.2E之间。 我们收集了P2、I222、P4和P41212表格中的数据集， 我把注意力集中在P1形式上，尽管它几乎是 总是孪生的，是最可复制的。 我们收集了数据 至2.10 E。 分析 自旋转函数和细胞的合理溶剂含量 （a=91 E，B=110 E，c=118 E，a=98，_=90，？= 96）指出， 细胞含有四种独特的分子。