DERIVATIVE & COMPLEX DATA COLLECTION FOR DNA HELICASE

衍生物

• 批准号: 6119411
• 负责人: CHRISTOPHER D PUTNAM
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119411
• 关键词: biomaterials; genome

We have ruled out a large number of heavy atom compounds and are focusing on techniques to specifically target heavy atoms to our crystals through surface systeine mutants like Tma RuvB N32C and by binding mercury to the non-hydrolyzable ATP analog ATPyS. We are excited about the preliminary analysis of the ATPgS data which suggests that significant conformational changes are occuring in the crystal as the data does not merge with wild type but two different ATPyS-Hg crystal soaks merged well with each other. We plan to recollect the ATPyS-HG and a wildtype ATPyS control to solve the structure of the nucleotide bound crystal. We are also working on cocrystallization experiments to limit crystal mosaicity.

我们已经排除了大量的重原子化合物， 专注于专门针对重原子的技术， 晶体通过表面系统突变体，如Tma RuvB N32 C， 将汞与不可水解的ATP类似物ATPyS结合。 我们 对ATPgS数据的初步分析感到兴奋， 表明，显着的构象变化发生在 晶体作为数据不与野生型合并，而是两个不同的 ATPyS-Hg晶体浸泡液彼此融合良好。 我们计划 选择ATPyS-HG和野生型ATPyS控件以解决 核苷酸结合晶体的结构。 我们还在研究 共结晶实验以限制晶体镶嵌性。