BACTERIAL RUVB DNA HELICASE

细菌 RUVB DNA 解旋酶

• 批准号: 6322190
• 负责人: CHRISTOPHER D PUTNAM
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6322190
• 关键词: biomaterials; genome; nucleic acids; structural biology

We plan to conduct some pilot experiments to collect the low resolution reflections on one (or more, depending on time) crystal forms of the bacterial RuvB DNA helicase. The helicase forms a hexameric ring with 32 point group symmetry and a number of crystal forms have substantial non-crystallographic symmetry. We hope to try to use some of the phasing techniques that use very low resolution reflections in combination with non-crystallographic symmetry averaging. The results of the data collection at SSRL will be used in combination with phasing models that will either be generated based on measurements of the dimensions of the helicase ring from electron microscopy or from electron microscopy of the crystals themselves in collaboration with Dr. Mark Yeager at The Scripps Research Institute.

我们计划进行一些试点实验，以收集低 一个（或多个，取决于时间）晶体上的分辨率反射 细菌RuvB DNA解旋酶的形式。 解旋酶形成 具有32点群对称性和多个晶体六聚环 形式具有实质上的非晶体学对称性。 我们希望尝试 使用一些使用非常低分辨率的定相技术 反射与非晶体学对称性结合 平均 SSRL的数据收集结果将用于 与相位模型相结合，这些相位模型将基于 解旋酶环尺寸的测量 显微镜或电子显微镜的晶体本身， 与Scripps研究所的Mark Yeager博士合作。