CRYSTAL STRUCTURES OF HUMAN POLYMERASE BETA COMPLEXED W/ DNA & NUCLEOTIDES

我们建议使用同步辐射和旋转相机 光束线7-1收集完整的衍射数据 人类 DNA 聚合酶 β (pol _) 与 DNA 复合的晶体 模板-ddNTP 不匹配。 Pol _ 是一种真核 DNA 聚合酶 参与受损DNA的修复。 具体来说，pol &Beta 有 已被证明参与碱基切除修复（BER）途径。 其较小的尺寸 (39 kD) 使得 pol &Beta 成为相对简单的 核苷酸转移反应机理研究的目标。 需要高分辨率数据才能看到构象的细节 变化以及构象变化如何提高保真度。

We propose to use synchrotron radiation and the rotating camera on beam line 7-1 to collect a complete set of diffraction data on crystals of human DNA polymerase beta (pol _) complexed with DNA template-ddNTP mismatches. Pol _ is a eukaryotic DNA polymerase involved in the repair of damaged DNA. Specifically, pol &Beta has been shown to participate in the base excision repair (BER) pathway. Its small size (39 kD) makes pol &Beta a relativelly uncomplicated target for mechanistic studies of the nucleotidyl transfer reaction. High resolution data is needed to see the detail of conformational changes and how conformational changes promote fidelity.