LASER MICROSURGERY ON MEIOTIC CRANE FLY SPERMATOCYTES: TELOMERES, KINETOCHORES

鹤蝇精母细胞减数分裂的激光显微手术：端粒、动粒

• 批准号: 6119669
• 负责人: JAMES LAFOUNTIAN
• 金额: $ 0.85万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119669
• 关键词: Mammalia; bacteria; bioimaging /biomedical imaging; biomedical resource; health care; human tissue; infection; microscopy

(Supported by NSF MCB 9808290 to J. LaFountain) This project involves using time-lapse video LM to follow the behavior of chromosome fragments created at various stages of meiosis in living crane fly spermatocytes by laser microsurgery. The laser is used to sever chromosomes into fragments that contain kinetochores, telomeres, or fragments that lack both of these structures. The behavior of these various fragments is then followed in vivo by video LM. The goal is to characterize the non-kinetochore based transport properties of meiotic spindles, and to compare these properties with those already known for plants and animal somatic cells. Preliminary data on this project that was obtained by Dr. LaFountains during his visits in 1997 has been used to obtain NSF support for an expanded effort. As a result he will be spending the next 18 months at the BMIRR facility, on sabbatical leave, pursuing the specific aim of his grant most of which involve laser microsurg ery on cr ane fly spermatocytes. Obviously, this work must be conducted at our facility because we have the only DIC based laser microsurgery system in the Nation. Last year this work was abstracted as: LaFountain, J. R., R.W. Cole and C. L. Rieder. (1997) Laser microsurgery of anaphase chromosomes in crane-fly spermatocytes: Kinetochore-independent movement of telomere-containing chromosome fragments. Molec. Biol. Cell 8:171a.

(由美国国家科学基金会MCB 9808290至J.LaFountain提供支持)此项目 包括使用延时视频LM来跟踪 减数分裂不同阶段产生的染色体片段 鹤飞精母细胞的激光显微手术。激光被用来 将染色体切成含有动粒、端粒、 或者是缺少这两种结构的碎片。的行为 这些不同的片段随后在活体中被视频LM跟随。这个 目标是表征基于非动粒的传输特性 减数分裂纺锤体的特性，并将这些特性与 已知的植物和动物体细胞。初步数据 关于拉方丹博士在访问期间获得的这个项目 1997年已被用来获得国家科学基金会的支持，以扩大努力。 因此，他将在接下来的18个月里在BMIRR度过 设施，正在休假，追求他的赠款的具体目标 其中大部分涉及激光对苍蝇精母细胞的显微手术。 显然，这项工作必须在我们的设施中进行，因为我们有 国内唯一的基于DIC的激光显微外科手术系统。去年 这项工作被抽象为：LaFountain，J.R.，R.W.Cole和C.L. 瑞德。(1997)晚期染色体的激光显微外科手术 鹤-蝇精母细胞：动粒非依赖性运动 含有端粒的染色体片段。莫莱克。比奥尔。8号牢房：171a。