IMPROVEMENTS IN RAPID MIX FLOW CELL DESIGN FOR SUB MILLISECOND PROTEIN FOLDING

亚毫秒级蛋白质折叠快速混合流动池设计的改进

• 批准号: 6205741
• 负责人: NEBOJSA MARINKOVIC
• 金额: $ 2.8万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205741
• 关键词: aging; animal tissue; biomedical resource; spectrometry

Osteoporosis is a disease characterized by reduction in bone mass, resulting in a fragile skeleton. It is clear that bone loss occurs in the disease; however, the quality of bone remodeled after the onset of Hthe disease is unknown. We are using synchrotron infrared microspectroscopy to analyze the chemical composition of bone remodeled before versus after the onset of osteoporosis. In our study, female cynomolgus monkeys were ovariectomized or sham-ovariectomized. After ovariectomization, many monkeys developed osteoporosis. After surgery, the monkeys were administered fluorescent drugs every six months for two years prior to necropsy. These fluorochromes (tetracycline, calcein, and xylenol orange) are taken into newly remodeled bone tissue. Thus, by combining fluorescence microscopy and infrared microspectroscopy, we are able to identify and compare the chemical composition of new versus older bone Hgrowth. We are examining the mineral/matrix ratios, mineral composition, and matrix composition as a function of bone age.

骨质疏松症是一种以骨量减少为特征的疾病， 导致骨骼脆弱。 很明显，骨质流失发生在 疾病；然而，骨质量在发病后发生了重塑 该病未知。 我们使用同步加速器红外 显微光谱分析骨的化学成分 骨质疏松症发病前与发病后的重塑。 在我们的 研究中，雌性食蟹猴被切除卵巢或 假卵巢切除术。 切除卵巢后，许多猴子发育 骨质疏松症。 手术后，猴子接受了 尸检前两年内每六个月使用一次荧光药物。 这些荧光染料（四环素、钙黄绿素和二甲酚橙）是 被吸收到新重塑的骨组织中。 因此，通过结合 通过荧光显微镜和红外显微光谱，我们能够 识别并比较新骨与旧骨的化学成分 生长。 我们正在检查矿物质/基质比率、矿物质 成分和基质成分作为骨龄的函数。